Browsing by Author "Ambilikutty Amma, P."
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Item Cocoa sweating, a promising culture medium for mass production of fungal bioagents(2011) Ambilikutty Amma, P.; Chandra Mohanan, R.Cocoa sweating collected on the first day of fermentation of beans was used for the preparation of culture media. When cocoa sweating as collected from the fermentary was used as liquid medium to grow Trichoderma harzianum and Metarhizium anisopliae, there was no growth. Therefore, growth and sporulation of both the fungi in different dilutions of cocoa sweating were compared with that in potato dextrose broth (PDB) and potato jaggery broth (PJB). Growth and sporulation of both the fungi were the highest in cocoa sweating (CS) diluted with tap water (TW) in the proportion CS: TW 1:4 (CS 1:4). Both the biocontrol agents did not exhibit any difference in growth and sporulation in CS 1:4 and in CS1:4 supplemented with 15 g dextrose and pH adjusted to 6.5. Growth and sporulation of both the fungi in CS1:4, PDB and PJB increased with number of days of incubation and was the highest on 20 th day after inoculation. When 800 ml CS 1:4 was used to grow the biocontrol agents and to prepare talc formulations using 2 kg talc powder, the moisture content was 8 % after 72 h of drying. Shelf life of talc formulations of both the biocontrol agents could be maintained for 5 months with very high colony forming units(CFUs). The CFUs /g of talc formulations of both the fungi stored for 6 months were less than that in the 5th month.Item Production Technology of Mycological Culture Medium Using Cocoa Bean Shell(2011) Ambilikutty Amma, P.; Chandra Mohanan, R.