Browsing by Author "Anishkumar, V.K."

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    Physiological Studies on Major Fungi of Leaf Rot Disease of Coconut
    (2005) Jyoti Rahna, S.; Anishkumar, V.K.; Srinivasan, N.
    Influence of culture media, pH, temperature and sources of carbon, and nitrogen on growth of major fungi of coconut leaf rot - Colletotrichum gloeosporioides, Exserohilum rostratum and Fusarium solani - was investigated in vitro. Potato dextrose agar was found to be best for the growth of all the three fungi. Czapek dox agar, glucose nitrate agar and Sabouraud dextrose agar were other favourable media for C. gloeosporioides and E. rostratum; Czapek dox agar to F. solani. Carrot agar and oat meal agar were less favourable for the growth of the fungi. In liquid media, high mycelia yield of C. gloeosporioides and E. rostratum occurred in potato dextrose broth, oat meal broth and Czapek dox broth and F. solani in oat meat broth. Carrot broth was least favourable for all the three fungi. Higher growth of C. gloeosporioides and F. solani occurred in the pH range of 6.0-6.5 while E. rostratum preferred a slightly more acidic pH. Higher growth of all the fungi occurred in the temperature range of 25°C-300C. Mannitol provided good growth of C. gloeosporioides in solid medium; fructose, glycerol, sorbitol and glucose were the other, next in order of preferred carbon sources for the fungus. Mannitol and sorbitol were favourable for the growth of E. rostratum. Glycerol was good to F. solani, besides other carbon sources such as sorbitol, glucose and fructose in influencing the fungal growth. In liquid medium also sorbitol and glucose were the preferred carbon sources, besides mannitol, by all the three fungi. All the three fungi preferred to grow best in sodium nitrate as the source of nitrogen both in solid and liquid media, followed by potassium nitrate, as compared to other sources of nitrogen tested. These findings would be helpful in understanding the physico-cultural behaviour of the fungi and also in context of their growth requirements.
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    Physiological Studies on Major Fungi of Leaf Rot Disease of Coconut
    (2005) Jyoti Rahna, S.; Anishkumar, V.K.; Srinivasan, N.
    Influence of culture media, pH, temperature and sources of carbon, and nitrogen on growth of major fungi of coconut leaf rot - Colletotrichum gloeosporioides, Exserohilum rostratum and Fusarium solani - was investigated in vitro. Potato dextrose agar was found to be best for the growth of all the three fungi. Czapek dox agar, glucose nitrate agar and Sabouraud dextrose agar were other favourable media for C. gloeosporioides and E. rostratum; Czapek dox agar to F. solani. Carrot agar and oat meal agar were less favourable for the growth of the fungi. In liquid media, high mycelia yield of C. gloeosporioides and E. rostratum occurred in potato dextrose broth, oat meal broth and Czapek dox broth and F. solani in oat meat broth. Carrot broth was least favourable for all the three fungi. Higher growth of C. gloeosporioides and F. solani occurred in the pH range of 6.0-6.5 while E. rostratum preferred a slightly more acidic pH. Higher growth of all the fungi occurred in the temperature range of 25°C-300C. Mannitol provided good growth of C. gloeosporioides in solid medium; fructose, glycerol, sorbitol and glucose were the other, next in order of preferred carbon sources for the fungus. Mannitol and sorbitol were favourable for the growth of E. rostratum. Glycerol was good to F. solani, besides other carbon sources such as sorbitol, glucose and fructose in influencing the fungal growth. In liquid medium also sorbitol and glucose were the preferred carbon sources, besides mannitol, by all the three fungi. All the three fungi preferred to grow best in sodium nitrate as the source of nitrogen both in solid and liquid media, followed by potassium nitrate, as compared to other sources of nitrogen tested. These findings would be helpful in understanding the physico-cultural behaviour of the fungi and also in context of their growth requirements.