Browsing by Author "Anitha Karun"

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    Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera
    (ScienceDirect, 2009) Moumita Chakraborty; Anitha Karun; Adinpunya Mitra
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    Application of RAPD markers in hybrid verification in coconut
    (2014) Rajesh, M.K.; Jerard, B.A.; Preethi, P.; Regi Jacob Thomas; Anitha Karun
    Coconut palms are classified into two major types, viz., ‘talls’ and ‘dwarfs’, which mainly differ in their pollination behavior of cross- and self-pollination, respectively. Due to this difference, getting true-to-type progenies of desirable tall and dwarf cultivars has always been a challenge. The conventional practice of selection of seedlings based solely on morphological traits often results in selection of out-crossed seedlings and undesirable off-types. In the present investigation, RAPD markers for the tall/dwarf trait were identified in coconut using a bulked DNA approach. Screening of tall and dwarf palm bulk DNA with 200 primers revealed a RAPD primer OPBA3 which was able to clearly differentiate both the tall and dwarf bulks. For validation, the primer was used to screen individual tall and dwarf coconut palms representing different geographic regions. The primer was also used to screen the parents and validate hybrids of Dwarf x Tall crosses.
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    Arecanut
    (2017) Anitha Karun; Krishna Prakash; Rajesh, M.K; Chowdappa, P
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    Arecanut
    (Today & Tomorrow's Printers and Publishers, New Delhi -110 002, India, 2017) Ananda, K.S.; Anitha Karun; Parthasarathy, V.A.
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    Assessment of genetic fidelity of arecanut plantlets derived through direct somatic embryogenesis by RAPD markers
    (Indian Society for Plantation Crops, 2008) Anitha Karun; Radha, E.; Sangeetha Vijayan, P.; Jiji George; Rajesh, M.K.; Ananda, K.S.
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    Authentication of coconut hybrids using RAPD analysis
    (2012-11) Rajesh, M.K.; Preethi, P.; Jerard, B.A.; Regi Jacob Thomas; Anitha Karun
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    Biotechnological achievements in plantation crops
    (2007-02-08) Nair, M.K.; Anitha Karun
    The conventional breeding techniques in improving plantation crops are slow and time-consuming, because of their iong juvenile phase, heterozygous outcrossing nature, and large area needed for their experimentation. Biotechnology can only be considered as a supplementary new tool to solve specific problems. Generally the plantation crops are beset with a narrow genetic base, lack of an inbred population, adequate land for field testing the selections and hybrids for yield and quality characters. The most popular and widely commercialized global application of biotechnology has been in the sphere of plant tissue culture— micropropagation. In India, only micropropagation in cardamom has been successfully commercialized among plantation crops.
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    Biotechnological Approaches
    (2017) Anitha Karun; Rajesh, M.K.; Muralikrishna, K.S.; Sajini, K.K.; Chowdappa, P.
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    Characterization of Annur and Bedakam Ecotypes of Coconut from Kerala State, India, Using Microsatellite Markers
    (2014-02) Rajesh, M.K.; Samsudeen, K.; Rejusha, P.; Manjula, C.; Shafeeq Rahman; Anitha Karun
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    Characterization of gibberellin 2-oxidase isoforms in coconut (Cocos nucifera L.)
    (2015-12) Shafeeq Rahman; Gangaraj, K.P.; Amal Vasu; Anitha Karun; Rajesh, M.K.
    Gibberellins (GAs) are plant hormones that are essential for many developmental processes in plants, including seed germination, stem elongation, leaf expansion, trichome development, pollen maturation and the induction of flowering. Gibberellin 2-oxidase (GA2-ox) regulates plant growth by inactivating endogenous bioactive GAs through 2β-hydroxylation. There is no information about GA2-ox encoding genes or their functions in coconut. In this study, we have identified 10 transcripts encoding different isoforms of GA2-ox from coconut leaf transcriptome data. Sequence comparison and phylogenetic analysis revealed that these 10 transcripts represented different types of GA2-ox. The secondary structure, three dimensional structure and active sites of these 10 isoforms were predicted. Docking studies of different active GAs with these isoforms was also carried out.
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    Cocoa
    (2017) Anitha Karun; Aparna, V.; Muralikrishna, K.S; Rajesh, M.K
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    Cocoa
    (2017) Anitha Karun; Aparna, V.; Rajesh, M.K; Chowdappa, P
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    Coconut
    (2017) Anitha Karun; Sajini, K.K.; Aparna, V.; Rajesh, M.K
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    Coconut
    (2017) Anitha Karun; Muralikrishna, K.S; Rajesh, M.K; Chowdappa, P
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    Coconut
    (2011) Rajesh, M.K.; Niral, V.; Anitha Karun; Parthasarathy, V.A.
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    Coconut (Cocos nucifera L.) Pollen Cryopreservation
    (2014) Anitha Karun; Sajini, K.K.; Niral, V.; Amarnath, C.H.; Remya, P.; Rajesh, M.K.; Samsudeen, K.; Jerard, B.A.; Florent Engelmann
    BACKGROUND: Coconut genetic resources are threatened by pests and pathogens, naturalhazards and human activities. Cryopreservation is the only method allowing the safe and costeffectivelong-term conservation of recalcitrant seed species such as coconut. OBJECTIVE: The objective of this work was to test the effect of cryopreservation and of cryostorage duration on coconut pollen germination and fertility. MATERIALS AND METHODS: Pollen of two coconut varieties (West Coast Tall WCT and Chowghat Orange Dwarf COD ) was collected in March-May over three successive years, desiccated to 7.5% moisture content (FW) and cryopreserved by direct immersion in liquid nitrogen. RESULTS: Germination and pollen tube length (PTL) of desiccated and cryopreserved pollen were not significantly different for both WCT and COD over the three harvest months of the three consecutive years of study. Pollen germination ranged from 24 to 32% in desiccated pollen whereas it was between 26 and 29% in cryopreserved COD pollen. In the case of WCT, germination ranged from 30 to 31% in desiccated pollen, while it was between 28 and 32% in cryopreserved pollen. PTL of cryopreserved pollen ranged between 224-390 m and 226-396 m for COD and WCT, respectively. Germination of COD pollen varied between 29.0 and 44.1% after 4 years and 1.0/1.5 years cryostorage, respectively. Germination of WCT pollen did not change significantly between 0 and 6 years cryostorage, being comprised between 32 (24 h) and 40 % (1.5 years). Germination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved. Normal seed set was observed in COD and WCT palms using pollen cryostored for 6 months and 4 years. Cryopreserved pollen of five Tall and five Dwarf accessions displayed 24-31% and 25-49% germination, respectively. CONCLUSION: These results show that it is now possible to establish pollen cryobanks to contribute to coconut germplasm long-term conservation.
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    Coconut Based Farming System: A Gandhia harmony of diverse crops, livestock and soil microorganisms
    (2020-10) Murali Gopal; Alka Gupta; Subramanian, P.; Anitha Karun
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    Coconut Biotechnology
    (2018) Rajesh, M.K; Anitha Karun; Parthasarathy, V.A.; p