Browsing by Author "Anuradha Upadhyay"

Now showing 1 - 14 of 14
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    Coconut genetic resources - Genetic and molecular approaches
    (2007-02-08) Ratnambal, M.J.; Khumaran, P.M.; Arunachalam, V.; Niral, V.; Anuradha Upadhyay; Parthasarathy, V.A.
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    Comparitive evaluation of embryo culture protocols in coconut
    (2007-02-08) Anitha Karun; Sajini, K.K.; Anuradha Upadhyay
    Four coconut embryo culture protocols, developed from PCA, Philippines, UPLB, Philippines, IRHO, France and CPCRI, India were tried with two tall and two dwarf cultivars. There was no significant difference among the protocols for per cent embryos germinated. However, difference among the cultivars and cultivar-by-protocol interaction was significant. The in vitro growth was slow in CPCRI protocol but the survival of the plants and ex vitro establishment were good. Vitrification was noticed only in liquid medium. Least number of plants was retrieved through IRHO protocol.
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    Effect of antioxidants on oil palm leaf in vitro
    (2007-02-08) Anuradha Upadhyay; Utpala Parthasarathy; Seema, G.; Anitha Karun; Parthasarathy, V.A.
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    An efficient method of DNA extraction from coconut leaves
    (2007-02-08) Anuradha Upadhyay; Parthasarathy, V.A.; Seema, G.; Anitha Karun
    A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion.
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    An efficient method of DNA extraction from coconut leaves
    (1999) Anuradha Upadhyay; Parthasarathy, V.A.; Seema, G.; Anitha Karun
    A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion
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    An Efficient Method of DNA Extraction from Theobroma Cacao and Cola Nitida Leaves
    (2003) Anuradha Sane; Anuradha Upadhyay; Manimekalai, R.; Parthasarathy, V.A.
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    GA3 and ABA mediated effect on maturation and germination of zygotic embryos in coconut (Cocos nucifera L.)
    (2007-02) Anitha Karun; Sajini, K.K.; Anuradha Upadhyay; Parthasarathy, V.A.
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    Genetic relationship and diversity in Indian coconut accessions based on RAPD markers
    (2004) Anuradha Upadhyay; Jayadev, K.; Manimekalai, R.; Parthasarathy, V.A.
    Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic diversity and genetic relationship among coconut accessions. DNA from 81 palms representing 20 accessions, 15 Indian and 5 exotic, was used to amplify with 8 highly polymorphic primers. The 8 primers yielded 77 markers, with an average of 9.6 markers per primer. The within-accession genetic diversity ranged from 0.057 to 0.196. In general, tall accessions were more heterozygous as they had higher proportions of polymorphic bands and genetic diversity. The proportion of variation explained by within accession and between accession diversity was 0.58 and 0.42, respectively. Similarly exotic accessions exhibited more variation. Dwarfs from geographically distant regions did not cluster separately. Based on the similarity matrix, cluster and principal coordinate analysis was performed. A dendrogram of genetic relationship was obtained. The extent of genetic diversity and genetic relationship among accessions is discussed.
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    Improved protocol for AFLP analysis as a base for tagging root (wilt) resistance genes in coconut
    (2002) Rajesh, M.K.; Jayadev, K.; Chandrasekhar, A.; Anuradha Upadhyay; Devakumar, K.; Manimekalai, R.; Nair, R.V.; Parthasarathy, V.A.
    The root (wilt) disease of coconut is endemic in Southern districts of Kerala and the adjoining areas of Tamilnadu causing enonnous loss of nuts. Integrated management practice is the only suggested method to reduce its effect to some extent while development of resistant varieties will offer permanent solution, for which concrete breeding efforts are required. Considering Ute long life cycle of coconut, selection of resistant varieties through conventional methods will be time consuming and laborious. Molecular markers offer numerous advantages over markers traditionally used in plant mapping and selective breeding. AFLP (Amplified Fragment Length Polymorphism) is a versatile DNA fingerprinting technique for marker assisted selection which combines universal applicability with high powers of discrimination and reproducibility. In this preliminary ttudy for tagging root(wh6 resistkce genesiicoconut, AFLP parameters, geirunning a& silver staining conditions were optimized. Also, primer pairs detecting variations between resistant and susceptible palms were selected.
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    Metabolic Changes in Coconut Embryo Culture with Various Antioxidants
    (2010) Anitha Karun; Anuradha Upadhyay; Radha, E.; Parthasarathy, V.A.
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    Optimization of DNA amplification fingerprinting parameters in coconut (Cocos nucifera L.)
    (2004-12) Manimekalai, R.; Jeya Dev, K.; Anuradha Upadhyay; Devakumar, K.; Rajesh, M.K.; Parthasarathy, V.A.; Rajagopal, V.; Kumaran, P.M.
    DNA Amplification Fingerprinting (DAF) is a simple and powerful technique for genome analysis. It is being employed in various applications in a number of crops. For obtaining reproducible results from DAF technique, optimization of PCR parameters was done. 20 ng (2 ng/ µl) template DNA, 15 µM primer, 250 µM each of dNTPs, 2 U (0.2 U/µl) Taq polymerase, 4 mM MgCl2, and annealing temperature of 55 "C, with standard silver staining protocol gave good amplification with reproducibility.S ubsequently these results will be used in tagging resistance genes for root (wilt) disease in coconut. The effect of GC content of primers was also discussed.
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