Browsing by Author "Asuncion P. Miniano"

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    Responses Of Non-Makapuno Embryos In Vitro To Chloride Supplementation
    (2007) Asuncion P. Miniano; Ernerita V. de Guzman
    Coconut embryos were cultured following a liquid-solid sequence. The media for the liquid culture were modified to provide increasing levels of chloride using NaCl, KC1 and NH4.Cl. The effect of the chloride was also tested by modifying the chlorine content of the medium for both the Liquid and solid cultures by substituting MgCI , for MgSO4- Growth of the embryos during the liquid culture was significantly enhanced by KC1 at 4x level but highly inhibited by NH.C1 at S\\ level. All leveis of KC! pretreatments significantly improved root development during the solid culture. Applied during the initial phase, MgCi . enhanced growth of the embryos in liquid culture and promoted leaf formation during the subsequent solid culture. Treatments during the liquid phase influenced growth and development during the subsequent solid culture. The lack of significant promotion of growth with NaCl and NH4C1 was attributed to the adverse effects of the accompanying cations.