Browsing by Author "Balachandran, S.M."

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    Clonal multiplication of oil palm (Elaeis guineensis Jacq)
    (2007-02-08) Raju, C.R.; Sajini, K.K.; Balachandran, S.M.; Saji, K.V.; Geetha Maheshan, K.; Rajasekharan, P.E.; Geetha, L.; Bavappa, K.V.A.
    Five mm long tender leaf explants and leaf base explants taken from 3-year-old seedlings were cultured on defined media supplemented with various auxins and cytokinins. Cultures were incubated in the light. The expaints produced callus from the veins at the cut surface in 2-3 weeks on R medium containing 20 mgl-1 2,4-D and 0.1 mgl"1 BAP. Somatic embryoids developed on lowering the 2,4-D concentration and replacing it with NAA. Bipolar and tripolar embryoids originated as snow-white protuberences from die sub-surface layers of the callus. The process of germination of the somatic embryoids was similar to that of normal zygotic embryos. However, the formation of adventitious roots was enhanced with die addition of 0.01 mgl-1 IBA to the medium.
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    Development of clonal plantlets from immature panicles of cardamom
    (2007-02-08) Kumar, K.B.; Prakash Kumar, P.; Balachandran, S.M.
    Cultured immature panicles of cardamom formed plantlels directly without the intervention of callus or embryoids, on MS medium supplemented with 0.5 mg/1 NAA, O.Smg/1 kinetin, 1.0 mg/1 BAP, 0.1mg/l calcium pantothenate, 0.1 mg/1 folic acid and 10% coconut water. The stage of development of the explant and the growth regulators in the medium are the two major factors influencing the frequency of shoot formation in the immature panicle cultures. The transformation occurs due to the reversal of morphogenesis in the floral primordia into vegetative shoots.