Browsing by Author "Balakrishnan, A."

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    Fatty acids in coconut oil
    (2006-09) Naresh Kumar, S.; Balakrishnan, A.; Rajagopal, V.
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    Quality and Fatty Acids of Coconut Oil Under Different Conditions and Duration of Storage
    (2012) Naresh Kumar, S.; Balakrishnan, A.
    Unrefined coconut oil is susceptible to oxidative and microbial rancidity, leading to objectionable flavour and reduction in shelf-life and resulting in economic loss. To analyse the fatty acid profile and free fatty acids (FFAs) in coconut oil during storage, oil was stored in different containers viz. transparent glass bottles, transparent plastic bottles, opaque bottles and brown glass bottle, and treated with additives viz., NaCl (1%), citric acid (100 ppm) and tamarind (5%). The fatty acid profile and FFA content in oil were estimated in each treatment at monthly interval for five months of storage. The results indicate that during storage, the small and medium chain fatty acids are degraded to form the FFAs. Additives such as citric acid (100 ppm) or common salt- NaCl (1%) prolonged shelf-life with least increase in FFAs. The study recommends that addition of NaCl to oil can be a better practice for storing oil instead of addition of tamarind, a traditional household practice.
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    Stress responsive proteins in coconut seedlings subjected to water, high-light, flooding and high-temperature stresses
    (2007-12) Naresh Kumar, S.; Kasturi Bai, K.V.; John George; Balakrishnan, A.; Siju Thomas, T.
    Coconut seedlings of different cultivars and cross combinations were subjected to stresses like water, high temperature and flooding stresses independently. Results indicated increase in the concentration of heat stable protein fraction (HSPF) in leaf tissue even when the total protein concentration reduced due to stress. The percentage of HSPF in leaf tissue increased with decrease in leaf water potentials. Quantitative changes in proteins also we& observed in leaf and root tissue due to temperature and flooding. In HSPF, proteins of -66KDa are found in root and leaf tissue. Leaflet tissues have specific in the range ~10 and ~14 KDa which are not present in the root tissues. Two extra proteins 66 KDa and ~76 KDa appeared in water stressed WCT seedlings. They were also present in seedlings exposed to high light intensity (~1,500 mmol/m2/S) apart from an extra LMW HSP of 14.4 KDa. Among the MMW- proteins, protein of 53 KDa is present in all WCT samples. The LMW protein of 20.1 KDa, present in non-stress seedlings disappeared during water stress period. In temperature induced and flooded leaflet tissue, new proteins (LMW range ~17 KDa) were observed. In temperature induced root tissue, a protein band in the range of 30 KDa was observed but not in flooded root or in control root. Results indicate quantitative and qualitative variations in stress proteins in coconut seedlings subjected to different abiotic stresses.