Browsing by Author "Belen F. Paloma, M."
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Item Storage and transport of zygotic embryos of Cocos nucifera L for in vitro culture(2007-02-08) Erlinda P. Rillo; Belen F. Paloma, M.Embryo culture of coconuts can circumvent problems of storage and transport caused by the weight and bulk of the nuts and the absence of dormancy. This paper describes a technique that can be routinely used for collecting large numbers of embryos. The embryos were extracted in the field, brought to a simple isolation room, washed in ethyl alcohol, disinfected in 1OO% commercial bleach for 20 minutes and then rinsed again. Sterilized embryos were sealed in sterile plastic bags and then transported in cold storage. Redisinfection in 100% commercial bleach for 5 minutes prior to culture under aseptic conditions was necessary to reduce contamination rates. This procedure did not adversely affect embryo germination.