Browsing by Author "Bhavyashree, U."
Now showing 1 - 17 of 17
Results Per Page
Sort Options
Item Comparative study of three different methods of coconut plumule extraction for embryogenic callus induction(2012-11) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut(2016) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Muralikrishna, K.S.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Cryopreservation of Cocoa Shoot Tips Using Pre Growth Desiccation Protocol(2011) Nilosha; Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem Effect of brassinosteroid, glutathione and adenine sulphate on callogenesis of coconut (Cocos nucifera L.) plumule(2012-11) Fayas, T.P.; Jeeha Muneem; Lakshmi Jayaraj, K.; Bhavyashree, U.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Effect of Culture Media and Explants on Callogenesis of Cocoa(2011) Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem Histological studies of cellular differentiation during somatic embryogenesis of coconut plumule-derived calli(2015-12) Lakshmi Jayaraj, K.; Bhavyashree, U.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunSince coconut is one of the most recalcitrant species to generate in vitro, it is necessary to study in detail about the cellular changes that occur during somatic embryogenesis to enhance our knowledge about this phenomenon. In the present study, coconut plumular tissues, the shoot meristem including leaf primordia, were used as explants for in vitro regeneration studies. Histological studies were carried out in different stages of plumule culture. No noticeable growth was observed in 15 days old cultures. After 30 days, meristematic cells could be identified. Abundance of meristematic cells, foremost to the development of callus structures, was observed after 45 days. After 75 days, globular friable calli were formed and histological studies revealed the presence of meristematic centers which eventually formed somatic embryos. The histological study of matured somatic embryos formed after 120 days of callus initiation showed a clear meristematic zone of parenchyma cells, surrounded by vascular bundles. Histological studies, carried out for certain abnormalities like compact calli, abnormal somatic embryoids with rudimentary shoots and multiplied roots, revealed the presence of intact cotyledonary leaves which seemed to inhibit the apical meristem development of somatic embryoids. The presence of vascular bundles in the early stages of callus formation might lead to the direct formation of meristemoids. These results could aid future studies leading to enhanced control of the somatic embryogenic process and greater efficiency of somatic embryo and plantlet formation in coconut.Item Histological studies of somatic embryogenesis of coconut plumule derived callus(2012-11) Lakshmi Jayaraj, K.; Bhavyashree, U.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Histological Studies on Callogenesis of Cocoa(2011) Bhavyashree, U.; Radha, E.; Anitha KarunItem Histological Studies on Callogenesis of Cocoa(2011) Bhavyashree, U.; Radha, E.; Anitha KarunItem In Vitro Multiplication of Cocoa(2011) Anitha Karun; Bhavyashree, U.Item Initiation of coconut cell suspension culture from shoot meristem derived embryogenic calli: A preliminary study(2016) Bhavyashree, U.; Lakshmi Jayaraj, K.; Muralikrishna, K.S.; Sajini, K.K.; Rajesh, M.K.Item Maintenance of embryogenic potential of calli derived from embryonic shoot of West Coast Tall cv. of coconut (Cocos nucifera L.)(2015) Bhavyashree, U.; Lakshmi Jayaraj, K.; Rachana, K.E.; Muralikrishna, K.S; Sajini, K.K.; Rajesh, M.K; Anitha KarunItem De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing(2016) Rajesh, M.K.; Fayas, T.P.; Naganeeswaran, S.; Rachana, K.E.; Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing(2016) Rajesh, M.K.; Fayas, T.P.; Naganeeswaran, S.; Rachana, K.E.; Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing(2016) Rajesh, M.K.; Fayas, T.P.; Naganeeswaran, S.; Rachana, K.E.; Bhavyashree, U.; Sajini, K.K.; Anitha KarunProduction and supply of quality planting material is significant to coconut cultivation but is one of the major constraints in coconut productivity. Rapid multiplication of coconut through in vitro techniques, therefore, is of paramount importance. Although somatic embryogenesis in coconut is a promising technique that will allow for the mass production of high quality palms, coconut is highly recalcitrant to in vitro culture. In order to overcome the bottlenecks in coconut somatic embryogenesis and to develop a repeatable protocol, it is imperative to understand, identify, and characterize molecular events involved in coconut somatic embryogenesis pathway. Transcriptome analysis (RNA-Seq) of coconut embryogenic calli, derived from plumular explants of West Coast Tall cultivar, was undertaken on an Illumina HiSeq 2000 platform. After de novo transcriptome assembly and functional annotation, we have obtained 40,367 transcripts which showed significant BLASTx matches with similarity greater than 40 % and E value of ≤10−5. Fourteen genes known to be involved in somatic embryogenesis were identified. Quantitative real-time PCR (qRT-PCR) analyses of these 14 genes were carried in six developmental stages. The result showed that CLV was upregulated in the initial stage of callogenesis. Transcripts GLP, GST, PKL, WUS, and WRKY were expressed more in somatic embryo stage. The expression of SERK, MAPK, AP2, SAUR, ECP, AGP, LEA, and ANTwere higher in the embryogenic callus stage compared to initial culture and somatic embryo stages. This study provides the first insights into the gene expression patterns during somatic embryogenesis in coconut.Item De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing(2016) Rajesh, M.K.; Fayas, T.P.; Naganeeswaran, S.; Rachana, K.E.; Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem Plantlet regeneration through indirect shoot organogenesis and somatic embryogenesis in Justicia gendarussa Burm. f., a medicinal plant(2013) Bhagya, N.; Chandrashekar, K.R.; Anitha Karun; Bhavyashree, U.A protocol for the regeneration of a large number of plantlets via indirect shoot organogenesis and somatic embryogenesis has been developed from the stem and leaf explants of Justicia gendarussa Burm. f. The callus was efficiently induced from the explants using Murashige and Skoog (MS) medium supplemented with α-Naphthalene acetic acid (NAA) + Benzyl amino purine (BAP) (1.0+ 0.1 mg/l). The highest number of plantlets through indirect shoot organogenesis was obtained when the callus was subcultured to MS medium with BAP + NAA (0.1+1.0 mg/l). The maximum number of plantlets via somatic embryos was obtained in the medium with BAP + NAA (1.0+0.1 mg/l) for stem derived calli and Kinetin (Kn) + NAA (2.0+ 0.1 mg/l) for leaf derived calli. The in vitro developed shoots were rooted well in half strength MS medium supplemented with 0.5 mg/l of Indole-3-acetic acid (IAA). The in vitro regenerated plantlets were hardened using a mixture of sterile sand:soil:manure (1:1:1). The present study is the first report on the regeneration of plants through somatic embryogenesis from stem and leaf derived calli of J. gendarussa.