Browsing by Author "Bobby Paul"

Now showing 1 - 4 of 4
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    CMSD: A Database of Microsatellite Markers Genotyped in Indigenous Coconut Accessions
    (2010) Bobby Paul; Rajesh, M.K.; Jayakrishnan, V.P.; Ramesh Kumar, M.P.; George V. Thomas
    The increased use of molecular markers in varietal identification has led to the need for an appropriate storage place and this has paved way for the development of a marker database containing allelic data of coconut accessions as revealed by microsatellite markers. The main features of the information system are a SSR database, SSR analysis tool, variety identification tool, data submission form and brief information about microsatellites. The database contains details of 11200 allelic data of 400 palms comprising 62 indigenous coconut accessions analyzed using 14 microsatellite primer pairs. Variety identification tool contains a display of 14 pairs of allelic entry corresponding to the 14 microsatellite primers. The result pages display the information about the query sample matches to a reference sample in the database and the score values they attain. Based upon the maximum score value, it can be concluded that the unknown sample is genetically related to the particular reference sample. The SSR allelic size data have been used for creating a DNA barcode system too, providing a more convenient handling of numerical data. This identification system with data warehouse can be accessed from the home page of Bioinformatics Centre, Central Plantation Crops Research Institute (ICAR), Kasaragod (http://www.bioinfcpcri.org/cmsd/home.php).
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    A comparative study of insect odorant protein binding (OBP) genes and isolation of a practical OBP gene from coconut red palm weevil rhynchophorus ferrugineus F.
    (2008-12) Rajesh, M.K.; Subaharan, K.; Ritto Paul; Sathish Kumar, R.; Bobby Paul; Sreesmitha, V.; George V. Thomas
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    In silico prediction of function and modelling of WRKY2 protein in coconut
    (2010-08) Sreesmitha, V.; Rajesh, M.K.; Bobby Paul; Anitha Karun; Ramesh Kumar, M.P.; George V. Thomas
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    Molecular cloning and characterization of a partial mitogen-activated protein kinase cDNA, CnMAPK1 induced in response to water stress in coconut
    (2012-06) Bobby Paul; Rajesh, M.K.; Naresh Kumar, S.
    Plants have developed a fine-tuned complex of stress responses, among which one of the earliest signalling pathways after perception of stimuli is the evolutionary conserved mitogen-activated protein kinase (MAPK) cascade. These cascades are major pathways by which extracellular signals are transduced into internal responses. In this study, oligonucleotide primers were designed based on conserved regions of plant MAPK sequences available in pubic databases. A cDNA clone of 467 bp encoding a MAPK (designated CnMAPK1) was isolated by RT-PCR from leaves of coconut plantlets subjected to water stress using polyethylene glycol (PEG) treatment. CnMAPK1 was significantly homologous to other plant MAPKs viz., MPK7 and MPK5 from Zea mays, SIPK and MAPK6 from Oryza sativa and FLRS from Triticum aestivum (93% identity). Coconut MAPK belongs to the Serine/Threonine Kinases (STKs), Plant TEY MAPK subfamily group A. Molecular modeling of the coconut MAPK was also undertaken.