Browsing by Author "Chen, J.T."

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    Morphogenetic routes of long-term embryogenic callus culture of Areca catechu
    (2010) Wang, H.C.; Chen, J.T.; Chang, W.C.
    Early morphogenetic events and repetitive embryogenesis from callus culture of betel nut palm (Areca catechu L.) were studied using scanning electron microscopy. On Murashige and Skoog (MS) medium supplemented with 2 mg dm-3 dicamba, callus culture has capacity to form plantlets via somatic embryogenesis and to form secondary embryos for about 4 years. However, various abnormal embryos without differentiation of the leaf sheath and shoot apical meristem were observed, which showed bell-shaped and then cup-shaped or mushroom-shaped structures. These abnormal embryos contained distinctive structures, including a disk-shape interior region, surfaces with grooves and a stalk-like posterior region. During subculture, these abnormal embryos enlarged, became deformed and gradually lose their shape and then converted into nodular, compact embryogenic callus. It was also found that secondary embryos originated from interior surfaces or posterior regions of abnormal embryos, and gave rise to the next cycle of normal and abnormal embryos.
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    Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu
    (BIOLOGIA PLANTARUM, 2006) Wang, H.C.; Chen, J.T.; Chang, W.C.
    Plant regeneration through somatic embryogenesis of Areca catechu L. was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations 2, 4, 6 and 8 mg dm-3 in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg dm-3 dicamba and during subculture on 2 - 8 mg dm-3 2,4-D or 2 - 4 mg dm-3 dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The plants grew well when transplanted to containers in shaded greenhouse