Browsing by Author "Doan Trong Duc"

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    Micropropagation of zedoary (Curcuma zedoaria Roscoe) – a valuable medicinal plant
    (Springer, 2007) Nguyen Hoang Loc; Doan Trong Duc; Tae Ho Kwon; Moon Sik Yang
    Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l)1 sucrose and 5 g l)1 agar, supplemented with 20% (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l)1. The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20% (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20% (v/v) CW, 3 mg l)1 BA, and 0.5 mg l)1 IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30–40 mm in length) were rooted on MS medium containing 20 g l)1 sucrose and 8 g l)1 agar, supplemented with 20% (v/v) CW and 2 mg l)1 NAA. More than 95% of the rooted plants were established in pots after hardening.