Browsing by Author "Emerita V. De Guzman"

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    The growth of coconut "Makapuno" embryos in vitro as affected by mineral composition and sugar level of the medium during the liquid and solid cultures
    (2007-02-08) Aurora G. Del Rosario; Emerita V. De Guzman
    Excised coconut makapuno embryos were sequentially cultured from liquid to solid medium using modified Whites basal medium and Murashige and Skoogs medium with varying levels of dextrose and other supplements. A low salt and high sugax concentration (Wo 8D) in the initial liquid medium and a high salt and moderate sugar concentration in the first transplant medium (Mo 4D) was found to be optimum for a balanced shoot and root development. During the liquid culture not all of the sugar supplied at S% was used up metabolically; a portion of it was active only as an osmotic agent. In the first transplant medium dextrose seems to be required solely as nutrient source.