Browsing by Author "Gangaraj, K.P."
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Item Assembly and Annotation of the Nuclear and Organellar Genomes of a Dwarf Coconut (Chowghat Green Dwarf) Possessing Enhanced Disease Resistance(2020) Rajesh, M.K.; Chowdappa, P.; Santosh Kumar Behera; Sandeep Kasaragod; Gangaraj, K.P.; Chinmaya Narayana KotimooleItem Characterization of gibberellin 2-oxidase isoforms in coconut (Cocos nucifera L.)(2015-12) Shafeeq Rahman; Gangaraj, K.P.; Amal Vasu; Anitha Karun; Rajesh, M.K.Gibberellins (GAs) are plant hormones that are essential for many developmental processes in plants, including seed germination, stem elongation, leaf expansion, trichome development, pollen maturation and the induction of flowering. Gibberellin 2-oxidase (GA2-ox) regulates plant growth by inactivating endogenous bioactive GAs through 2β-hydroxylation. There is no information about GA2-ox encoding genes or their functions in coconut. In this study, we have identified 10 transcripts encoding different isoforms of GA2-ox from coconut leaf transcriptome data. Sequence comparison and phylogenetic analysis revealed that these 10 transcripts represented different types of GA2-ox. The secondary structure, three dimensional structure and active sites of these 10 isoforms were predicted. Docking studies of different active GAs with these isoforms was also carried out.Item Characterization, Structural Modeling and Docking Study of CnCNLR1, A CC-NBS-LRR Protein from Coconut(2018) Rachana, K.E.; Gangaraj, K.P.; Rajesh, M.KItem The complete chloroplast genome data of Areca catechu (Arecaceae)(2020) Rajesh, M.K.; Gangaraj, K.P.; SudheeshK. Prabhudas; T.S. Keshava PrasadItem The complete chloroplast genome data of Areca catechu (Arecaceae)(2020) Rajesh, M.K.; Gangaraj, K.P.; Sudheesh K. Prabhudas; Keshava Prasad T.S.Item Dataset of dual RNA-sequencing of Phytophthora palmivora infecting coconut ( Cocos nucifera L.)(2020) Gangaraj, K.P.; Rajesh, M.K.Item Development of EST‑SSR markers for genetic diversity analysis in coconut (Cocos nucifera L.)(2020) Preeti P; Shafeeq Rahman; Naganeeswaran, S.; Sabana, A.A.; Gangaraj, K.P.; Jerard, B.A; Niral, V.; Rajesh, M.K.Item Draft genome sequence, annotation and SSR mining data of Oryctes rhinoceros Linn. (Coleoptera: Scarabaeidae), the coconut rhinoceros beetle(2021) Rajesh, M.K.; Ginny Antony; Kumar Arvind; Jeffrey Godwin; Gangaraj, K.P.; Sujithra, M; Joseph Rajkumar, A.; Tony GraceItem An improved device for coconut embryo extraction(2017-12) Devakumar, K.; Neema, M.; Aparna, V.; Krishna Prakash; Gangaraj, K.P.; Anitha KarunItem In vitro assay for screening of Areca spp. for Phytophthora resistance(2018) Muralikrishna, K.S.; Sharadraj, K.M; Gangaraj, K.P.; Nagaraja, N.R.; Anitha Karun; Rajesh, M.K.; Chowdappa, P.Item Isolation and characterization of partial secA gene from coconut root wilt and arecanut yellow leaf disease phytoplasma and assessment of its possible use for sero diagnosis(2015-12) Manimekalai, R.; Smita Nair; Gangaraj, K.P.; Soumya, V.P.; Rao, G.P.A 425 bp fragment of phytoplasma secA gene encoding SecA, a component of the Sec protein translocation system, was isolated and characterized from coconut root wilt disease and arecanut yellow leaf disease phytoplasma, both belonging to the 16SrXI·B sub group phytoplasma. The gene showed 99% nucleotide identity with sugarcane grassy shoot phytoplasma secA gene. In the phylogenetic analysis of the translated SecA protein sequence, all the 16SrXI group phytoplasmas clustered together. The three dimensional structure of the protein was predicted using Moddler 9.13. The validation of the predicted structure indicated that 83.6% of the amino acids lie in the favored region indicating that the predicted structure was ideal. The possible antigenic domains in the protein were also predicted using the tool SVM TriP. The antigenicity prediction of the SecA protein fragment showed the presence of 4 possible antigenic domains. The secA gene was expressed in pET vector and the protein was used for the development of polyclonal antiserum in rabbit. Because the titre ot the phytoplasma was very less, the color development in ELISA was less Intense. A technique for isolation of higher number of phytoplasma cells has to be standardized.Item Molecular characterization of phytoplasma associated with phyllody of Pedalium murex - a common weed in coconut plantations(2015) Merin Babu; Josephrajkumar, A.; Rajumon, M.; Devika, S.; Rajeev, G.; Gangaraj, K.P.; Vinayaka HegdeItem Virtual screening and in vitro evaluation of potential growth regulators against somatic embryogenesis receptor- like kinase(SERK) in Cocos nucifera L.(2015) Rajesh, M.K.; Gangaraj, K.P.; Jamshinath, T.P.; Fayas, T.P.; Naganeeswaran, S.; Sajini, K.K.; Anitha Karun