Browsing by Author "Giuseppe Genchi"

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    Dietary fatty acid composition differently influences retinoylation reaction in rat testes mitochondria
    (2007) Paola Tucci; Valentina Senatore; Erika Cione; Francesco Genchi; Anna M. Giudetti; Gabriele V. Gnoni; Giuseppe Genchi
    All-trans-retinoic acid (atRA) is incorporated covalently into proteins of rat testesmitochondria. In this study, the effect of three diets with different fatty acid composition on the retinoylation of proteins of rat testes mitochondria has been investigated. Different groups of rats were fed on a basal diet supplemented with 15% of either coconut oil (CO), olive oil (OO) or fish oil (FO).We found that,when compared with CO, the binding of retinoic acid was decreased in FO- and OO-fed rats. Mitochondrial phospholipids composition was differently influenced by dietary treatments; minor changes were observed in fatty acid composition of phospholipids. Few differences were observed in the Arrhenius plots among the three groups of rats. Kinetic analysis revealed a decrease in the Vmax value in FO- and OO- as compared with CO-fed rats. No difference among the three groups were observed in the KM value. The retinoylation reaction was inhibited by 13-cis-RA and 9-cis-RA.