Browsing by Author "Hanold, D."
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Item Coconut cadang-cadang viroid infection of African oil palm(2009-02) Randles, J.W.; Rodriguez, J.M.B.; Hanold, D.; Vadamalai, G.Item Detection of coconut cadang-cadang viroid-like sequences in oil and coconut palm and other monocotyledons in the south-west Pacific(1991) Hanold, D.; Randles, J.W.Item Localisation of coconut foliar decay virus in coconut palm(2007-02-08) Randles, J.W.; Hanold, D.; Rohde, W.; Morin, P.; Miller, D.C.Coconut foliar decay virus (CFDV) occurs at a very low concentration in coconut palm. A 1203 nucleotide segment of the scquenced encapsulated circular single-stranded 1291 nucleotide CFDV-DNA has been amplified and transcribed for use as a p cDNA probe for the virus. A rapid method for the extraction of DNA from coconut palm has been devised for a dot-blot hybridisation assay using this probe. An alternative non-radioactive probe has also been developed for future use in CFDV diagnosis. CFDV-DNA was shown to be distributed unevenly in a range of infected palms, necessitating the use of multiple sampling to reliably detect infection in diagnostic tests. Viral DNA was detected in symptomatic and asymptomatic palms of both high and low susceptibility, in disease-free tolerant cultivars. and in palms in remission from disease. Within the same palm, detectability of viral DNA varied little within leaflets, but varied more within and between fronds, CFDV-DNA was detected 6-8 months after insect-mediated inoculation, and symptoms generally appeared after another 1-4 months. In sun hybridisation of rachis tissue showed localisation of DNA within the phloem. bin its distribution in the phloem was uneven. CFDV-DNA was detected in tissue adjacent to and within necrotic zones which develop into the petiolar lesions associated with the disease-specific collapse of fronds. Virus was detected in the body of the insect vector, and, where its distribution could be resolved, in the abdomen rather than the head.Item The use of cloned sequences for the identification of coconut foliar decay disease associated DNA(1988) Hanold, D.; Langridge, P.; Randles, J.W.Item The use of cloned sequences for the identification of coconut foliar decay disease associated DNA(1988) Hanold, D.; Langridge, P.; Randles, J.W.Item Variants of coconut Cadang-cadang Viroid Isolated from an African Oil Palm (elaies Guineensis Jacq.) In Malaysia(Springer-Verlag, 2006-07) Vadamalai, G.; Hanold, D.; Rezaian, M.A.; Randles, J.W.Variants of coconut Cadang-cadang Viroid have been Identified in a Plantation Oil Palm Growing in Malaysia. Three Size Classes are Described, Comprising 297, 293, and 270?nt. Compared with the 296-nt Form of Coconut Cadang-cadang Viroid (cccvd), all Variants Substituted C31???u in the Pathogenicity Domain and A175???c in the Right-hand Terminus. Other Mutations and Deletions Accounted for the Different Sizes. These are the first Sequences Reported for Variants of coconut Cadang-cadang Viroid in a Species other than Coconut Palm, and This is the first Evidence that Variants Closely Related to Cccvd Occur Outside the Philippines.