Browsing by Author "Jeya Dev, K."

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    Optimization of DNA amplification fingerprinting parameters in coconut (Cocos nucifera L.)
    (2004-12) Manimekalai, R.; Jeya Dev, K.; Anuradha Upadhyay; Devakumar, K.; Rajesh, M.K.; Parthasarathy, V.A.; Rajagopal, V.; Kumaran, P.M.
    DNA Amplification Fingerprinting (DAF) is a simple and powerful technique for genome analysis. It is being employed in various applications in a number of crops. For obtaining reproducible results from DAF technique, optimization of PCR parameters was done. 20 ng (2 ng/ µl) template DNA, 15 µM primer, 250 µM each of dNTPs, 2 U (0.2 U/µl) Taq polymerase, 4 mM MgCl2, and annealing temperature of 55 "C, with standard silver staining protocol gave good amplification with reproducibility.S ubsequently these results will be used in tagging resistance genes for root (wilt) disease in coconut. The effect of GC content of primers was also discussed.