Browsing by Author "Jongedijk, E."
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Item A Quick Enzyme Squash Technique For Detailed Studies On Female Meiosis In Solanum(1987-05) Jongedijk, E.Item A rapid methyl salicylate clearing technique for routine phase-contrast observations on female meiosis in Solanum(1987-05) Jongedijk, E.A rapid clearing technique, involving methyl salicylate clearing and phase-contrast microscopy, that enables large-scale observations on meiosis and embryo sac development in intact ovules of potato, was developed. With high resolution phase-contrast optics the details observed in cleared ovules were as well defined as in microtome sections and 'abnormalities' associated with desynapsis and 2n-megaspore formation were readily detected. In potato this ovule clearing technique offers certain advantages over earlier clearing and staining-clearing techniques and permits bulk preparation of ethanol (70%) stored ovaries shortly (1-5-2 h) before observation.