Browsing by Author "Krajinski, F."

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    Mtha1, a Plasma Membrane H+-ATPase Gene from Medico truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue
    (2002-12) Krajinski, F.; Hause, B.; Gianinazzi-Pearson, V.; Frankon, P.
    Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H+-ATP-ases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H+-ATPases were identified in the genome of Medicago truncatulo and three cDNA fragments of the l-T-ATPase gene family (Mthol-3) were obtained by RT-PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha2 and Mtha3 appeared to be con-stitutively expressed in roots and unaffected by AM development, transcripts of Mthal could only be detected in AM tissues and not in controls. Further analyses by RT-PCR revealed that Mtha~\ transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that /Wthol RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule-specific induced expression of a plant H+-ATPase gene in mycorrhizal tissues.