Browsing by Author "Kularatene, J.D.J.S."

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    Effect of Gelling Agent and Selective Sub-culturing on Hyperhydricity in Anther-Derived Coconut Embryos
    (2011) Perera, P.I.P.; Kularatene, J.D.J.S.; Weerakoon, L.K.
    Experiments were conducted to compare the liquid medium with the media solidified with agar or phytagel.Selective subculturing and use of the embryo maturation medium supplemented with higher concentration of phytagel(0,5%; w/v) were also tested for reducing the vitrified embryos,Modified Eeuwens Y3 medium was used as the basal medium. By culturing the anthers on the medium solidified with phytagel (0.25%; w/v), direct embryo formation (86 .7%) and embryo conversion(21.5%) were significantly increased. Plant regeneration efficiency of anther derived embryos or calluses developed in the liquid culture medium was extremely low (2.4%) . Vitrification was further reduced by incorporating 0.5% (w/v) phytagel into the embryo maturation medium. Highest plant regeneration efficiency was obtained by exposing the embryos to 0.5%(w/v) phytagel for 21 days,which reduced vitrification by 42%. Furthermore, selective subculturing of the embryos was effective for reducing vitrification.