Browsing by Author "Kuzelova, M."

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    Hypercholesterolemia Abrogates an Increased Resistance of Diabetic Rat Hearts to ischemia-reperfusion Injury
    (Springer-Verlag, 2007) Adameova, A.; Kuzelova, M.; Andelova, E.; Faberova, V.; Pancza, D.; Svec, P.; Ziegelhoffer, A.; Ravingerova, T.
    Both, Diabetes Mellitus (dm) and Hypercholesterolemia (hch) are Known as Risk Factors of Ischemic Heart Disease, However, the Effects of Experimental Dm, as well as of Hch Alone, on ischemia/reperfusion-induced Myocardial Injury are not Unequivocal.We have Previously Demonstrated an Enhanced Resistance to ischemia-induced Arrhythmias in Rat Hearts in the Acute Phase of Dm.Our Objectives were thus to Extend our Knowledge on how Dm in Combination with Hch, a Model that is Relevant to Diabetic Patients with Altered Lipid Metabolism, may Affect the Size of Myocardial Infarction and Susceptibility to Arrhythmias.A Combination of Streptozotocin (stz;80 Mg/kg, I.P.) and the fat-cholesterol Diet (1% Cholesterol, 1% Coconut Oil;Fchd) was Used as a double-disease Model Mimicking Dm and Hch Simultaneosly Occurring in Humans.Following 5 Days after Stz Injection and Fchd Leading to Increased Blood Glucose and Cholesterol Levels, Anesthetized open-chest Diabetic, diabetic-hypercholesterolemic (DM-HCH) and age-matched Control Rats were Subjected to 6-min Ischemia (occlusion of Lad Coronary Artery) Followed by 10 Reperfusion to Test Susceptibility to Ventricular Arrhythmias in the in Vivo Experiments and to 30-min Ischemia and Subsequent 2-h Reperfusion for the Evaluation of the Infarct Size (is) in the Langendorff-perfused Hearts.The Incidence of the most life-threatening Ventricular Arrhythmia, Ventricular Fibrillation, was Significantly Increased in the DM-HCH Rats as Compared with non-diabetic Control Animals (100% Vs.50%;p<0.05).Likewise, Arrhythmia Severity Score (as) was Significantly Higher in the DM-HCH Rats than in the Controls (4.9±0.2 Vs. 3.5±0.5;p<0.05), but was not Increased in the Diabetic Animals (as 3.7±0.9;p>0.05 Vs.Controls).Diabetic Hearts Exhibited a Reduced is (15.1±3.0% of the Area at Risk Vs.37.6±2.8% in the Control Hearts; p<0. 05), However, a Combination of Dm and Hch Increased the Size of Myocardial Infarction to that Observed in the Controls. In Conclusion, Hch Abrogates Enhanced Resistance to ischemia-reperfusion Injury in the Diabetic Rat Heart.