Browsing by Author "Lakshmi Jayaraj, K."
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Item Assessment of cross-taxa utility of coconut microsatellite markers(2008-09) Anitha, N.; Lakshmi Jayaraj, K.; Arun Kumar, E.P.; Jiji George; Rajesh, M.K.Micro-satellite or simple sequence repeats (SSRs) are abundant across eukaryotic genomes and show high levels of polymorphism. Micro-satellite DNA often has flanking regions that are highly conserved in related species and this renders the primer pairs designed in one species useful for the amplification of the same DNA region in related genomes, minimizing laborious cloning and screening steps. The present investigation explored the transferability of coconut SSRs to other palms, viz., oil palm, arecanut, palmyrah and date palm. The annealing temperature of 86 coconut-specific SSR primers was standardized using gradient PCR. Of the 86 primers, 55 primers gave clear bands of expected size range (100 to 300 bp) and these were tested for their cross-taxa amplification. The percentage of cross-amplification of coconut SSR loci were 36.36% in oil palm, 29.09% in arecanut, 18.18% in palmyrah and 12.70% in date palm. The results suggest usefulness of coconut SSRs for phylogenetic and comparative genomic studies in other palms.Item Comparative study of three different methods of coconut plumule extraction for embryogenic callus induction(2012-11) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut(2016) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Muralikrishna, K.S.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Effect of brassinosteroid, glutathione and adenine sulphate on callogenesis of coconut (Cocos nucifera L.) plumule(2012-11) Fayas, T.P.; Jeeha Muneem; Lakshmi Jayaraj, K.; Bhavyashree, U.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Histological studies of cellular differentiation during somatic embryogenesis of coconut plumule-derived calli(2015-12) Lakshmi Jayaraj, K.; Bhavyashree, U.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunSince coconut is one of the most recalcitrant species to generate in vitro, it is necessary to study in detail about the cellular changes that occur during somatic embryogenesis to enhance our knowledge about this phenomenon. In the present study, coconut plumular tissues, the shoot meristem including leaf primordia, were used as explants for in vitro regeneration studies. Histological studies were carried out in different stages of plumule culture. No noticeable growth was observed in 15 days old cultures. After 30 days, meristematic cells could be identified. Abundance of meristematic cells, foremost to the development of callus structures, was observed after 45 days. After 75 days, globular friable calli were formed and histological studies revealed the presence of meristematic centers which eventually formed somatic embryos. The histological study of matured somatic embryos formed after 120 days of callus initiation showed a clear meristematic zone of parenchyma cells, surrounded by vascular bundles. Histological studies, carried out for certain abnormalities like compact calli, abnormal somatic embryoids with rudimentary shoots and multiplied roots, revealed the presence of intact cotyledonary leaves which seemed to inhibit the apical meristem development of somatic embryoids. The presence of vascular bundles in the early stages of callus formation might lead to the direct formation of meristemoids. These results could aid future studies leading to enhanced control of the somatic embryogenic process and greater efficiency of somatic embryo and plantlet formation in coconut.Item Histological studies of somatic embryogenesis of coconut plumule derived callus(2012-11) Lakshmi Jayaraj, K.; Bhavyashree, U.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Initiation of coconut cell suspension culture from shoot meristem derived embryogenic calli: A preliminary study(2016) Bhavyashree, U.; Lakshmi Jayaraj, K.; Muralikrishna, K.S.; Sajini, K.K.; Rajesh, M.K.Item Maintenance of embryogenic potential of calli derived from embryonic shoot of West Coast Tall cv. of coconut (Cocos nucifera L.)(2015) Bhavyashree, U.; Lakshmi Jayaraj, K.; Rachana, K.E.; Muralikrishna, K.S; Sajini, K.K.; Rajesh, M.K; Anitha Karun