Browsing by Author "M.K. Rajesh"

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    A rapid and simplified technique for inducing Ganoderma lucidum infections in coconut and arecanut seedlings
    (2026) Daliyamol; K.K. Greena; V.H. Prathibha; M. Amruthalekshmi; M.K. Rajesh; Y. Diwakar; Vinayaka Hegde
    Ganoderma lucidum is a soil borne fungi known to cause Basal stem rot (BSR) or Thanjavur wilt or Ganoderma wilt disease in coconut as well as Foot rot or Anabe roga disease in arecanut plantations. Due to its soil borne nature, effectively managing the disease in field conditions is highly challenging. To combat this issue, the development of resistant or tolerant seedlings is crucial for sustainable production of coconut and arecanut palms. Therefore, a reliable and rapid method to assess resistance of palms to Ganoderma disease is essential. Here we report a rapid inoculation technique wherein seedlings were soaked in 5 days old mycelia cultures supplemented with 0.002% Tween 20 for a period of 60 min. The initial symptoms could be noticed 15 days after
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    From the discovery of a novel arepavirus in diseased arecanut palms (Areca catechu L.) in India to the identification of known and novel arepaviruses in bee and plant transcriptomes through data-mining
    (2024) R. Thava Prakasa Pandian; Bhavishya; V. Kavi Sidharthan; M.K. Rajesh; Merin Babu; Susheel Kumar Sharma; B.J. Nirmal Kumar; M. Chaithra; Vinayaka Hegde
    Arecanut palm is a commercially important plantation crop valued for its nut. In this investigation, we report the discovery of a putative novel arepavirus, named areca palm necrotic ringspot virus 2 (ANRSV2), in necrotic ringspot diseased areca palms in Bantwal, Dakshina Kannada, Karnataka, India through RNA-sequencing and transmission electron microscopy. Further, the presence of ANRSV2 in the diseased samples was confirmed through reverse transcriptase-polymerase chain reaction assays. In addition, by mining public domain transcriptome data for arepaviral sequences, we identified a putative novel arepavirus in Psychotria rubra, a non-palm host. We recovered the genome sequences of the areca palm necrotic ringspot virus in honey bees, tomato, Onobrychis viciifolia, and Rhamnus heterophylla. These findings broaden our comprehension of arepaviral diversity and host range, and suggest an intriguing possibility of pollen-mediated arepaviral transmission that necessitates empirical validation. Further studies are needed to understand the biology of identified putative novel arepaviruses.