Browsing by Author "Marcio Gilberto Cardoso Costa"

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    Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
    (2008) Thiago Edson Ribeiro da Silva; Luciana Cardoso Cidade; Fatima Cerqueira Alvim; Julio Cezar de Mattos Cascardo; Marcio Gilberto Cardoso Costa
    The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specifi c. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.