Browsing by Author "Margarita Mauro-Herrera"

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    Ambiguous genetic relationships among coconut (Cocos nucifera L.) cultivars: the effects of outcrossing, sample source and size, and method of analysis
    (2010) Margarita Mauro-Herrera; Alan W. Meerow; Lalith Perera; Joanne Russell; Raymond J. Schnell;
    A prior analysis of eight coconut cultivars with 15 microsatellite (SSR) markers drew unexpected relationships between two of the out-crossing tall cultivars evaluated: ‘Atlantic Tall’ and ‘Panama Tall’. We further investigated the relationships between these eight cultivars by increasing the number of individuals studied (particularly for ‘Atlantic Tall’ and ‘Panama Tall’), by including 28 more molecular markers, and by adding two other cultivars to our analysis. Our results show that five to ten coconut individuals do not represent a dependable sample to withdraw conclusions regarding cultivar/variety relationships, particularly when studying out-crossing genotypes. As suggested in the prior study, a high level of hybridization was observed between the ‘Atlantic Tall’ and ‘Panama Tall’ cultivars. However, at this time we were able to identify distinct groups for each one of these two cultivars. The two clustering methods used (Neighbor Joining, NJ and Unweighted Pair Group Method with Arithmetic mean, UPGMA) produced dendrograms that resolved contrasting cultivar relationships, especially for the ‘Atlantic Tall’ and ‘Panama Tall’ cultivars. We discuss the implications of our results in regard to current scenarios of coconut domestication and future considerations when assessing genetic relationships among different varieties.
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    Ten informative markers developed from WRKY sequences in coconut in coconut (Cocos nucifera)
    (Blackwell Publishing Ltd, 2006) Margarita Mauro-Herrera; Alan W. Meerow; James W. Borrone; David N. Kuhn; Raymond J. Schnell
    Coconut ( Cocos nucifera L.) WRKY sequences containing single nucleotide polymorphisms (SNPs) and one microsatellite repeat were used to develop 10 informative markers. These markers were evaluated in 15 genotypes representing six coconut cultivars. SNP-containing alleles were detected by single-strand conformation polymorphism (SSCP) analysis. The number of detected alleles ranged from two to four. Five pairs of loci were in linkage disequilibrium in the test population. These markers are currently being evaluated in more individuals/ cultivars to determine their value in estimating the genetic diversity of this species.