Browsing by Author "Mathews T. Thelly"

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    Cloning and sequencing of phytoplasma ribosomal DNA(r DNA) associated with Kerala wilt disease of coconut palms
    (2004) Sharmila, L.B.; Salini Bhasker; Mathews T. Thelly; Boby T Edwin; Mohankumar, C.
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    H+-ATP ase - A biochemical marker for early detection of root (wilt) disease in coconut palms (Cocos nucifera L.)
    (2000-01) Mathews T. Thelly; Mohankumar, C.
    H+-ATPase, the key enzyme of secondary transport of tons, cell turgor changes ,and intracellular pi-E regulation showed maximum activity in leaf samples of healthy palms, whereas the palms of diseased area expressed a reduction in activity to a minimum level. Activity difference of H+-ATPase in healthy and diseased palms was further substantiated by detection of the enzyme by histochemical method.A decrease in activity of H+-ATPase was noticed in palms without any visual symptoms of the disease, otherwise called as apparently healthy, from the healthy palms of disease free area. This result indicates that, the palms of diseased area lacking visual symptoms could be identified biochemically by H+-ATPase activity. Physiological suppression of H+-ATPase in diseased palms was also supported by structural variations of leaf and root tissues observed under SEM.
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    H+ATPASE - A biochemical marker for early detection of root (wilt) disease in coconut palms (Cocos nucifera L.)
    (2007-02) Mathews T. Thelly; Mohankumar, C.
    H+-ATPase, the key enzyme of secondary transport of tons, cell turgor changes ,and intracellular pi-E regulation showed maximum activity in leaf samples of healthy palms, whereas the palms of diseased area expressed a reduction in activity to a minimum level. Activity difference of H+-ATPase in healthy and diseased palms was further substantiated by detection of the enzyme by histochemical method.A decrease in activity of H+-ATPase was noticed in palms without any visual symptoms of the disease, otherwise called as apparently heathy, from the healthy palms of disease free area. This result indicates that, the palms of diseased area lacking visual symptoms could be identified biochemically by H+-ATPase activity. Physiological suppression of H+-ATPase in diseased palms was also supported by structural variations of leaf and root tissues observed under SEM.
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    Suppression of plasma membrane H+- ATPase in root (wilt) diseased coconut palms (cocos nucifera l.) impairs stomatal opening and phloem transport
    (2001-01) Mathews T. Thelly; Sharmila, L.B.; Mohankumar, C.
    Plasma membrane H+-ATPase, couples ATP hydrolysis to proton transport thereby establishing the driving force for solute transport into and out of plant cells. A sound depletion of H+-ATPase activity was noticed in diseased palms from that of healthy ones. The suppression of plasma membrane H+-ATPase in diseased palm was further substantiated by the impaired functioning of stomata and the disintegration of phloem and tracheary elements as observed under Scanning Electron Microscope (SEM). The results indicate the functional imbalance of electro chemical gradient created by H+-ATPase in the leaf cells. Moreover, the undifferentiated shrunken leaf tissues of diseased palms clearly clarify the loss of turgidity and cell wall plasticity due to suppression of H+-ATPase