Browsing by Author "Mayilvaganan, M."
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Item Alterations in zymogram of peroxidase and esterase isoenzymes in leaves of root (wilt) diseased coconut (Cocos nucifera L.) cv. West Coast Tall(2007-02-08) Mayilvaganan, M.; Alka GuptaItem Alternations in zymogram of peroxidase and esterase isoenzymes in leaves of root(wilt) diseased coconut (Cocos nucifera L.) cv. West Coast Tall (2007-02-08) Mayilvaganan, M.; Alka GuptaItem Biochemical evidences for purification of phytoplasma of root (wilt) disease of coconut(2007-02-08) Mayilvaganan, M.; Alka Gupta; Chithra, K.R.Phytoplasma of root (wilt) disease of coconut (Cocos nucifera L.) has been purified from tender leaf tissues of diseased palms by Percoll-discontinuous density gradient centrifugation (PDDGC). Purified phytoplasma fractions were checked by biochemical methods such as ultraviolet-absorption spectrum (190-310 nm) and sodium dodecyl sulphate-polyacrylamide gel elcctrophoresis (SDS-PAGE). PDDGC produced a single turbid zone of phytoplasma at the top of 30% step gradient from diseased leaf samples, which was not seen in samples from healthy leaf. Phytoplasma from this fraction had an absorption maximum near 200 nm. On further fractionation, the protein and nucleic acid components of phytoplasma showed absorption peaks at 280 and 260 nm respectively. The protein of phyloplasma from diseased leaf samples produced three major peptides of 29, 28 and 18.5 kDa in SDS-PAGE.Item Changes in fat components and fatty acid compositions of autoxidized groundnut oils(2007-02-08) Mayilvaganan, M.; Singh, S.P.Item Chemical synthesis of ferrugineol and field assay for trapping Rhynchophorus ferrugineus Oliv.(2007-02-08) Mayilvaganan, M.; Nair, C.P.R.; Shanavas, M.; Saritha S. NairItem Comparison of different DNA isolation methods for PCR detection of coconut root (wilt) phytoplasma(Indian Society for Plantation Crops, 2006) Rintu Rebecca Jacob; Mayilvaganan, M.Item Developing a polymerase chain reaction based detection method for coconut root (wilt) phytoplasma(2007) Mayilvaganan, M.; Gunasekaran, M.; Sheenakumari, T.K.; Geetha, L.; Rajeev, G.; Solomon, J.J.; Sasikala, M.Item Effects of autoxidized groundnut oils on activities of digestive enzymes - lipase and pepsin(2007-02-08) Mayilvaganan, M.; Singh, S.P.Item Efficacy of low cost Pheromone Lure (Ferrugineol) against red palm weevil in coconut(2004-01) Rajamanickam, K.; Mayilvaganan, M.; Nair, C.P.R.Item Efficacy of low cost Pheromone Lure (Ferrugineol) against red palm weevil in coconut(The Andhra Agricultural Journal, 2004-01) Rajamanickam, K.; Mayilvaganan, M.; Nair, C.P.R.Item Electron microscopic evidence for purification of coconut root (wilt) disease phytoplasma(2007-02-08) Mayilvaganan, M.; Solomon, J.J.Root (wilt) disease phytoplasma was purified from diseased coconut tissues using discontinuous Percoll gradient centrifugation method. Crude sap prepared from coconut was layered on a discontinuous Percoll gradient of 15, 30, 50 and 60%(v/v). After centrifugation at 20.000 gfor 30 min. the turbid fraction formed on the top of 30% gradient in the diseased plant material was recovered, processed and fixed for electron microscopy. Electron microscopic examination of sections prepared from purified preparation of diseased plant material showed typical cells of root (wilt) phytoplasma with heterogeneous sizes and more or less spherical shape that are similar to those found in sieve elements of diseased tissues and salivary glands of infective (viruliforms) . insect vectors. These purified phytoplasma bodies showed trilaminar membrane with internal materials of ribosome granules and DNA fibrils. However, the yield in terms of number of cells was fewer and in addition to intact bodies, free membranes and empty bodies lacking internal contents also were observed.Item Evaluation of CPCRI red weevil pheromone lure in pollachi tract of Tamil Nadu(2006) Rajamanickam, K.; Mayilvaganan, M.; Nair, C.P.R.Item Field assay of locally synthesised ferrugineol for trapping Rhynchophorus ferrugineus(2003-03) Mayilvaganan, M.; Nair, C.P.R.; Shanavas, M.; Saritha S. NairItem An Ideal Method for Separation of Protein from Purified Coconut Root (Wilt) Phytoplasma(2007-02) Mayilvaganan, M.Separation of protein component from phytoplasma is important for its characterization and use as antigen for production of phytopiasma-specific antiserum, since proteins make the best antigens. Protein was isolated from purified phytoplasma of coconut root (wilt) disease by three methods and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Comparative analysis of different methods showed that the method in which water-saturated phenol with 8-hydroxyquinoline and 0.1% sodium dodecyl sulphate were used, yielded more undegraded and readily soluble proteins that were distinctly separated in SDS-PAGE. The other two methods wherein water-saturated phenol with 8-hydroxyquinoline and liquid phenol alone was used, gave much lower protein yield that were insoluble and degraded in electrophoresis. SDS-PAGE analysis of the proteins fractionated by the three methods showed that 2 bands resolving at 28 and 29 kDa were observed only in the case of phytoplasma proteins isolated by water-saturated phenol, 8-hydroxyquinoline and 0.1% SDS method, which were observed as a single band in the other two methods. These results thus indicate that the water-saturated phenol, 8-hydroxyquinoline and 0.1% SDS method is most suitable for separation of protein from purified phvtoplasma.Item Oleochemicals from Coconut Oil(2004-09) Mayilvaganan, M.Item Oleochemicals from Coconut Oil(2004) Mayilvaganan, M.Item Physico-chemical changes due to coreid bug infestation on coconut(Indian Society for Plantation Crops, 2002) Mayilvaganan, M.; Radhakrishnan Nair, C.P.Item Physico-chemical changes in tender coconut due to Paradasynus rostratus Dist. infestation(2006) Mayilvaganan, M.; Radhakrishnan Nair, C.P.Item Preparation of Coconut Root (wilt) Phytoplasma specific Antiserum and its use in Disease Detection(2007-02) Sasikala, M.; Solomon, J.J.; Chithra, K.R.; Rajeev, G.; Mayilvaganan, M.Item Refinement of ELISA and its use in early detection of coconut root (wilt) disease(2005) Sasikala, M.; Prakash, V.R.; Sapna, V.P.; Mayilvaganan, M.; Leena S. Nair