Browsing by Author "Nakamura, T."

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    Establishment of oil palm cell suspensions and plant regeneration
    (1995) Teixeira, J.B.; Sondahl, M.R.; Nakamura, T.; Kirby, E.G.
    Primary globular callus from immature zygotic embryos and friable embryogenic tissue derived from mature zygotic embryos were used to establish suspension cultures, Callus cultures were established either on modified Y3 or MS medium containing 475-500 microM 2,4-D or 250 microM picloram and 0.3 % (w/v) activated charcoal. Suspension cultures of both cell lines were established in modified Y3 medium containing 10 microM 2,4-D. The establishment of ccll suspensions from friable embryogenic tissue took only 2 months, in contrast with suspensions frol11 primary globular callus which took 3-5 months to establish. Embryo differentiation was observed only in cell suspensions derived from the friable embryogenic tissue after plating aliquots on regeneration medium. Germinated embryos were recovered and plantlets were successfully established under greenhouse conditions. Abbreviations: CET -compact embryogenic tissue, FET -Friable embryogenic tissue, ClM -callus induction medium . PGC -primary globular callus, 2.3-D -2,4-dichlorphenoxyacetic acid Y3 -Eeuwens' medium, MS-Murashige & Scoog medium. PYP-40 -polyvinylpyrrolidone, KM -Kao & Michayluk vitamins, ABA -abscisic acid