Browsing by Author "Neema, M."
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Item Basidiomycete (Pseudolagarobasidium acaciicola) in coconut (Cocos nucifera) suspension culture – a report.(2023) Neema, M.; Alka Gupta; Murali Gopal; Anitha KarunItem Coconut(2017) Neema, M.; Rajesh, M.K; Ramesh, S.V.; Chowdappa, PItem Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements(2017) Anitha Karun; Rajesh, M.K; Sajini, K.K.; Muralikrishna, K.S; Neema, M.; Shareefa, M.; Regi Jacob ThomasItem Double coconut - A unique giant palm from Seychelles(2020) Neema, M.; Aparna, V.; Krishna PrakashItem Effect of charcoal-impregnated calcium alginate spherules in mitigating polyphenolic interference in coconut (Cocos nucifera L.) suspension cultures.(2026) Neema, M.; Aparna, V.; Sudha, R.; Chandran, K.P.; Zaeema, F.; Sugatha, P; Ramesh, S.V.Phenolic oxidation and browning pose significant challenges in coconut cell suspension culture. Although activated charcoal is commonly used to mitigate phenolic interference, its powder form reduces medium clarity, thereby hindering precise quantification. The present study aimed to address this issue by developing an alternative method of incorporating activated charcoal that maintains its functional role while preserving culture transparency. For this, calcium alginate spherules of two sizes (4 and 6 mm) were prepared using varying concentrations of sodium alginate and calcium chloride, and their stability in suspension culture conditions was evaluated. Selected spherules were incorporated with activated charcoal and were further tested for polyphenol adsorption capacity in gallic acid solutions and coconut cell suspension cultures. While the spherules of treatment S1T20 (6 mm diameter, 3.0% sodium alginate with 0.4 M calcium chloride), incorporated with 2.0 g L− 1 of activated charcoal, adsorbed 68 to 80% of the polyphenols in coconut cell suspension, those devoid of activated charcoal exhibited no phenol adsorption. Proliferation of the cell and formation of cell clumps occurred in media with activated charcoal (either in the powder form or as incorporated in calcium alginate spherules), whereas the cells elongated and vacuolated in media without activated charcoal. This study introduced, for the first time, an effective alternative to the conventional approach of incorporating activated charcoal powder in suspension cultures using calcium alginate spherules. This approach effectively addressed the challenge of phenolic interference while simultaneously enhancing medium clarity facilitating accurate visualization and quantification of cultured cells. The proposed technology holds promise not only for coconut suspension cultures but also for application across a wide range of plant species where activated charcoal is essential during various stages of in vitro culture. The study provided important insights that can guide future research efforts to enhance the somatic embryogenesis protocol in coconut.Item Electrical Induction as Stress Factor for Callus Growth Enhancement in Plumular Explant of Coconut (Cocos nucifera L.)(2022) Neema, M.; G. S. Hareesh; Aparna, V.; Chandran, K.P.; Anitha KarunItem Enhancement of callogenesis from plumular explants of coconut (Cocos nucifera) via exogenous supplementation of amino acids and casein hydrolysate(2023) Aparna, V.; Neema, M.; Chandran, K.P.; Muralikrishna, K.S.; Anitha KarunItem An improved device for coconut embryo extraction(2017-12) Devakumar, K.; Neema, M.; Aparna, V.; Krishna Prakash; Gangaraj, K.P.; Anitha KarunItem An insight into pollen morphology and evaluation of pollen viability, germination and mineral composition of some coconut (Cocos nucifera L.) genotypes(2022) Sudha, R.; Niral, V.; Samsudeen, K.; Aparna, V.; Selvamani, V.; Neema, M.Item Nata de coco- A unique value added product from coconut(2016-04) Shameena Beegum; Neema, M.; Aparna, V.; Manikantan, M.R.Item Perspectives on the cardioprotective, neuroprotective and anti-obesity functions of coconut (Cocos nucifera L.)(2024) Shameena Beegum; Ramesh, S.V.; Pandiselvam, R.; Neema, M.; Daliyamol; Manikantan, M.R.; Hebbar, K.BWidely acclaimed as the "tree of life," the coconut is intrinsically woven into human culture as a source of food, shelter, and medicine. Coconut oil and its derivatives have found diverse applications in both food and industry. Of late, the consumption of coconut in the form of oil and its derivatives has been proven to be beneficial. Taking into account the most recent clinical evidences, an attempt was made to present a concise review of the cardioprotective, neuroprotective, and anti-obesity effects of coconut and its derivatives. Our analysis reveals that research evidence supports the cardioprotective and neuroprotective effects of coconut. Numerous clinical trials have proven the anti-obesity and hypoglycemic effects of coconut oil and products. High contents of myocardial anti-oxidants, and differential metabolism of medium chain fatty acids (MCFAs) provide cardioprotective effects. The ketogenic effect of coconut derived products confers neuroprotective measures and enhanced energy expenditure in the metabolism of MCFAs and polyphenolic anti-oxidants are suggested to offer anti-obesity effects. Nevertheless, further research with more randomized, controlled, large clinical trials that evaluate the optimal dosage and side effects, if any, are warranted. Based on this comprehensive review, it is understood that MCFAs are the key component, apart from the phytochemicals such as polyphenols, tocopherols, and other antioxidants that accord these health benefits. Overall, the health benefits of coconut and its derivatives are perceptible, though there is a need for long-term clinical trials. Also a shift of research focus from coconut fatty acids and oil to other phytochemicals and to design appropriate clinical and epidemiological studies to discover coconut biomolecules of health importance is warranted.Item Pre-sowing seed treatments for Indian indigo (Indigofera tinctorial(2018) Neema, M.; Aparna, V.; Krishna Prakash; Reghunath, B.R