Browsing by Author "Padmaja Haridasan"

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    Tissue Culture Studies in Turmeric
    (1982) Kuruvina Shetty, M.S.; Padmaja Haridasan; Iyer, R.D.
    Single sprouting buds of turmeric clone 15B were cultured on a modified Murashige and Skoog medium containing sucrose (40g/l), kinetin (0.2 to 0.5 mg/1), adjusted to pH 5.6 and gelled with 1 g/1 of agar agar. On subsequent transfer to the medium of same composition, these individual plants developed a marked swelling at the base. During the second month of culture, 5 to 25 shoots were produced from a single explant. At the point of swelling, a soft, translucent callus was produced. The callus could easily be separated from the developing plant. When subcultured and exposed to light, the callus grew further, simultaneously producing several buds that turned green and later developed into plantlets. The rate of multiplication was high and could be used for initial multiplication of the promising clones.