Browsing by Author "Pathmanathan Umaharan"
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Item Association mapping of seed and disease resistance traits in Theobroma cacao L(2016) Lambert A. Motilal; Dapeng Zhang; Sue Mischke; Lyndel W. Meinhardt; Michel Boccara; Olivier Fouet; Claire Lanaud; Pathmanathan UmaharanItem Cocoa(2017) Lambert A. Motilal; Antoinette Sankar; David Gopaulchan; Pathmanathan UmaharanItem Increasing Accuracy and Throughput in Large-Scale Microsatellite Fingerprinting of Cacao Field Germplasm Collections(2009) Lambert A. Motilal; Dapeng Zhang; Pathmanathan Umaharan; Sue Mischke; Stephen Pinney; Michel BoccaraIn this study we report on increasing the rate and accuracy of microsatellite fingerprinting of accessions in Theobroma cacao L. field germplasm collections with a medium-throughput capillary sequencing system. We examined the use of a reduced number of microsatellite loci to decrease the time and materials required for fingerprinting and determined the best available microsatellite loci for accurately separating accessions. A subset of nine informative loci that could separate sixty accessions into the same genetic groupings as a complete set of 37 loci was found. Stringent probability of identity values (i.e. chance of unique accession) was highly influenced (r=−0.996; P<0.001) by the number of alleles utilised in the fingerprinting set but the composition of the primer set was more important when choosing discriminatory loci. DNA pooling to reduce the number of samples was also investigated. There was a relatively high level of mixture within plots (59% of 54 plots examined) of the field genebank, which opposed the use of a pooling strategy to fingerprint the multiple trees of an accession plot in the collectionItem Proteomic and peptidomic UHPLC-ESI MS/MS analysis of cocoa beans fermented using the Styrofoam-box method(2020) Emanuele Scollo; David C.A. Neville; M. Jose Oruna-Concha; Martine Trotin; Pathmanathan Umaharan; Darin Sukha; Rena Kalloo; Rainer CramerItem The relic Criollo cacao in Belize - genetic diversity and relationship with Trinitario and other cacao clones held in the International Cocoa Genebank, Trinidad(2010) Lambert A. Motilal; Oapeng Zhang; Pathmanathan Umaharan; Sue Mischke; Vishnarayan Mooleedhar; Lyndel W. Meinhardt