Browsing by Author "Prabhu, K.V."

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    Genetics of resistance to stem rust against Indian races in wheat varieties, Kundan and UP 2338
    (2014-09) Nagaraja, N.R.; Anupam Singh; Pallavi, J.K.; Sharma, J.B.; Karnam Venkatesh; Singh, G.P.; Neelu Jain; Prabhu, K.V.
    The parents, F1 and F2 individuals of the crosses Kundan x Agra Local, UP 2338 x Agra Local and Kundan x UP 2338 were screened to study the genetics of resistance in the Ug99-resistant varieties of wheat, Kundan and UP 2338 against two Indian stem rust pathotypes 40A (62G29) and 122 (7G11). Against race 40A (62G29), the variety Kundan did not have any resistance alleles, whereas, UP 2338 exhibited the presence of dominant resistance gene. The 195 F2 individuals of the cross UP 2338 × Agra Local and Kundan × UP 2338 segregated at a ratio of 3(R):1(S). Against stem rust pathotype 122 (7G11), the variety Kundan displayed monogenic dominant resistance. The resistance x resistance cross Kundan x UP 2338 also displayed resistance reaction in the F2 generation with no segregation indicating the presence of common gene in both the parents (Kundan and UP 2338) conferring resistance against the pathotype 122.
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    Validation of molecular markers linked to the stem rust resistance genes effective in India
    (2013) Nagaraja, N.R.; Anupam Singh; Pallavi, J.K.; Sharma, J.B.; Singh, G.P.; Neelu Jain; Prabhu, K.V.
    An investigation was carried out to validate the already known molecular markers Xgwm533, Xcfa2019 & Xcfa2123, Sr24#12 & Sr24#50, Sr26#43 and BE518379, SCSS30.2 and Sr39-I & Sr39-II for stem rust resistance genes Sr2, Sr22, Sr24, Sr26, Sr31 and Sr39, respectively in wheat cultivars Kundan and UP 2338 carrying unknown stem rust resistance gene(s). The Sr2 and Sr22 gene specific SSR markers amplified the gene specific or expected allele in both Sr2 and Sr22 carrying and non-carrying cultivars. Sr24 associated STS markers (Sr24#12 & Sr24#50) amplified their respective expected fragments (500 bp and 200 bp) only in known Sr24 gene carrying cultivar, HD 2851. Two DNA markers (Sr26#43 & BE518379) known for Sr26 gene were screened. Marker, Sr26#43 amplified the specific fragment of 207 bp only in known source carrying Sr26 gene, while other marker, BE518379 functioned as null allele marker by not amplifying the critical band in Sr26 lines. SCAR marker, SCSS30.2 specific to Sr31 gene amplified the gene specific fragment in wheat cultivar UP 2338 confirming the presence of Sr31. Out of 14 entries tested for Sr39 gene including Kundan and UP 2338, the expected PCR product was not amplified in any of the entries indicating absence of Sr39 gene.