Browsing by Author "Quainoo, A.K."

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    Effect of Liquid Nitrogen Storage Time on the Survival and Regeneration of Somatic Embryos of Cocoa
    (2009) Quainoo, A.K.
    Investigations were undertaken on the effect of liquid nitrogen (LN) storage time on survival and regeneration of somatic embryos of cocoa (Theobroma cacao l.). Somatic embryos from different cocoa genotypes (AMAZ 3- 2, AMAZ 10-1, AMAZ 12, SIAL 93, and IMC 14) at 15.45% moisture content were cryopreserved in LN for one h, four and eight weeks. Somatic embryos of the genotypes emerged from the alginate beads at different periods 4 to 12 weeks post-cryopreservation. Individual genotypes subjected to low temperature storage time did not show significant differences in post-cryopreservation survival, although different genotypes responded differently with AMAZ 12 and IMC 14 recording the highest and lowest mean survival rates of 58% and 35%, respectively. Plantlets originating from five genotypes had been weaned and developed normally comparable to non-cryopreserved somatic embryo-derived plantlets in the glasshouse. The effects of low temperature storage time on survival and genetic stability of somatic embryos using genotypes from the same parental and distinct lines may contribute to long-term storage of cocoa germplasm.
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    The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees
    (2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
    Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSS V-infected Ameionado cocoa trees (T 1 , T2 and T4). The virps was transmitted to primary somatic embryos induced from the i nfecied callus tissues atiherace of 10 (J9%), 18 (14%) and 16 (15%) forTl.T2 and T4, re«,p
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    Transmission of cocoa swollen shoot virus by seeds
    (Elsevier, 2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
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    Transmission of cocoa swollen shoot virus by seeds
    (2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
    A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. FCR/captllary electrophoresis. using cocoa swollen shoot virus primers designed from the most conserved regions of the six published cocoa genome sequences, allowed the detection of cocoa swollen shoot virus in jil the component parts of cocoa seeds from cocoa swollen shoot virus-infected trees. PCR/capiHary electrop horesis revealed the presence of cocoa swollen shoot virus in seedlings raised from seeds obtained from cocoa swollen shoot virus-infected trees. The high frequency with which the virus was transmitted through the seedlings suggested that cocoa swollen shoot virus is transmitted by seeds. This has serious implications for cocoa germplasm conservation and distribution.