Browsing by Author "Rajesh, M.K"
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Item 5-Azacytidine promotes the induction of embryogenic calli and somatic embryos from transverse thin cell layer (tTCL) cultures in coconut(2024) Keezhath Thazha Kuniyil Amritha; Muralikrishna, K.S.; Jasmin Habeeb; Chandran, K.P.; Paulraj, S.; Rajesh, M.KCoconut (Cocos nucifera L.) is highly recalcitrant to in vitro interventions. There is a need to overcome various bottlenecks to standardize a repeatable protocol for in vitro regeneration in coconut to meet the requirements of quality planting materials. Epigenetic processes, especially DNA methylation, are known to assay crucial roles in regulating genes controlling plant growth and development, especially somatic embryogenesis. In this study, we demonstrate that the supplementation of 5-azacytidine (5-azaC), a demethylating agent, in the coconut tissue culture media can enhance the formation of embryogenic calli, somatic embryos, and plantlet regeneration from transverse thin sections of mature zygotic embryos. Transverse thin cell layer (tTCL) sections of zygotic embryos were cultured onto Y3 medium supplemented with different concentrations of 5-azaC (0, 10, 15, and 20 μM), auxins (2,4-D, picloram and atrazine; 75 and 100 μM) and thidiazuron (TDZ;4.5 μM). Explants were exposed to constant 5-azaC and reduced auxin concentration in subsequent sub-culturing. Our results indicated supplementing 15 μM 5-azaC, in combination with picloram (75 μM) and TDZ (4.54 μM), improved the percentage of callusing (95.8%) and formation of embryogenic calli (87.5%), and formation of somatic embryos (4.7) and plantlets (4.0) per explant in comparison with control having 80.8%, 75.0%, 1.6 and 0.67, respectively from tTCL sections of mature zygotic embryos. The results will form the basis for designing more efficient coconut tissue culture protocols.Item A new pod bioassay method to determine the toxicity of insecticides against Tea mosquito bug, Helopeltis theivora(2024) T. N. Madhu; Saneera, E.K; R. Thava Prakasha Pandian; Sujithra, M; Bhavishya; Nagaraja, N.R.; Elain Apshara, S.; Josephrajkumar, A.; Rajesh, M.KItem Arecanut(2017) Nagaraja, N.R.; Ananda, K.S.; Rajesh, M.KItem Arecanut(2017) Anitha Karun; Krishna Prakash; Rajesh, M.K; Chowdappa, PItem Artificial intelligence in plantation crops(2019) Chowdappa, P; Ravi Bhat; Mathew, A.C; Rajesh, M.KItem Assimilating socio-economic perspective in designing crop sector technology interventions: A farmer participatory study on coconut sector in Kerala(2019-12) Thamban, C.; Lijo Thomas; Chandran, K.P.; Jayasekhar, S.; Rajesh, M.K; Jesmi Vijayan; Srinivasan, V.; Nair, K.M.; Anil Kumar, K. S.Item Characterization, Structural Modeling and Docking Study of CnCNLR1, A CC-NBS-LRR Protein from Coconut(2018) Rachana, K.E.; Gangaraj, K.P.; Rajesh, M.KItem Cocoa(2017) Anitha Karun; Aparna, V.; Muralikrishna, K.S; Rajesh, M.KItem Cocoa(2017) Anitha Karun; Aparna, V.; Rajesh, M.K; Chowdappa, PItem Coconut(2017) Anitha Karun; Sajini, K.K.; Aparna, V.; Rajesh, M.KItem Coconut(2017) Anitha Karun; Muralikrishna, K.S; Rajesh, M.K; Chowdappa, PItem Coconut(2017) Neema, M.; Rajesh, M.K; Ramesh, S.V.; Chowdappa, PItem Coconut Biotechnology(2018) Rajesh, M.K; Anitha Karun; Parthasarathy, V.A.; pItem Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements(2017) Anitha Karun; Rajesh, M.K; Sajini, K.K.; Muralikrishna, K.S; Neema, M.; Shareefa, M.; Regi Jacob ThomasItem COCONUT(COCOS NUCIFERA L.)POLLEN CRYOPRESERVATION(2014) Anitha Karun; Sajini, K.K.; Niral, V.; Amarnath, C.H.; Remya, P.; Rajesh, M.K; Samsudeen, K; Jerard, A.; Florent EngelmannItem Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis(2018) Prathibha, V.H.; Vinayaka Hegde; Sharadraj, K.M; Rajesh, M.K; Rachana, K.E.; Chowdappa, PItem Identification and utilization of informative EST-SSR markers for genetic purity testing of coconut hybrids(2016-08) Preethi, .P; Rajesh, M.K; Rahul, C.U; Jerard, B.A.; Samsudeen, K; Regi Jacob Thomas; Anitha KarunCoconut palms are categorized into two forms, viz., ‘talls’ and ‘dwarfs’ which are being utilized to produce hybrids through the process of inter-varietal or intra-varietal crosses. Hybrid coconut seedlings are generally identified and selected based on morphological traits by plant breeders, which is quite difficult and requires expertise. Even minor errors in identification may adversely affect breeding programs in coconut, which is spread over many decades. In this study, we have utilized thirty EST-SSR markers, derived from existing coconut leaf transcriptome data, for screening polymorphism between eighteen coconut parental lines. The polymorphic primers capable of differentiating the parental palms were then utilized successfully for assessment of purity of hybrids derived from these parents. Thus, the current study demonstrates the utility of EST-SSR markers in determining the genetic purity of hybrids in coconut.Item In vitro regeneration of coconut plantlets from immature inflorescence(2019) Shareefa, M.; Regi Jacob Thomas; Sreelekshmi, J.S; Rajesh, M.K; Anitha KarunItem Insect Neuropeptides and Application in Pest Management(2018) Joseph Rajkumar, A.; Rajesh, M.K; Chowdappa, PItem Maintenance of embryogenic potential of calli derived from embryonic shoot of West Coast Tall cv. of coconut (Cocos nucifera L.)(2015) Bhavyashree, U.; Lakshmi Jayaraj, K.; Rachana, K.E.; Muralikrishna, K.S; Sajini, K.K.; Rajesh, M.K; Anitha Karun