Browsing by Author "Ranjitha Kumari, B.D."

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    Microsatellite Marker based Characterization of South Pacific Coconut (Cocos nucifera L.) Accessions
    (2011) Pradeep Kumar, S.; Manimekalai, R.; Ranjitha Kumari, B.D.
    The coconut palm (Cocos nucifera L.) is one of the major perennial oil crops of tropics providing the basis in many developing countries for food and industrial products. DNA based molecular marker techniques have proven powerful in genetic diversity estimation and it is advantages over morphological and biochemical markers. The aim of the present study was to identify the microsatellite primers (SSR) which are used to access genetic diversity among worldwide collection of germplasm (14 accessions). Assessment and distribution of genetic diversity was done with the help of software POPE GENE ver.1 and statistical parameters A total of 8 primers were used in the present study which produced 28 polymorphic alleles. The highest similarity index (0.7654) was observed between the accessions Hari papua Dwarf and kiriwana Tall the similarity coefficient ranged from 0.1775 to 0.7654 with a mean of 0.4231. The accessions grouped into three clusters in the dendrogram. Cluster 1 consisted of five accessions belonging to New Guinea. Cluster 2 consisted of four accessions belonging to French Polynesia. Cluster 3 consisted of five accessions belonging to Solomon Island were identified from south pacific coconut accessions.