Browsing by Author "Rema S. Soumya"

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    Isolation of beta-mannanase from Cocos nucifera Linn haustorium and its application in the depolymerization of beta-(1 ,4)-linked D-mannans
    (2010-05) Rema S. Soumya; Emilia Abraham, T.
    Beta mannanase was extracted from coconut(Cocos nucifera Linn) haustorium and purified through ammonium sulphate precipitation and sepharose 6B-lectin affinity chromatography.Coconut beta mannanase is an acidic protein with a pI of 3.75.The molecular mass of coconut beta mannanase (sodium dodecyl sulfate-polyacrylamide gel electophoresis) was found to be 44kDa and was confirmed by matrix-assisted lase desorption ionization time-of-flight mass spectrometry.the optimum temperature and pH for enzyme activity was 70c and 5.2.the enzyme was used for the preparation of neutraceutical dietary supplement from glactomannans of guar gum and tender coconut kernel having a beta-(1,4)-linked D-mannanose backbone.Depolymerised guar gum has 92% of oligosaccharides with a degree of polymerization of 9-39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize beta-(1,4)-linked D-mannose poly-saccharides from the most plant sources to produce prebiotics in a cost-effective technique.