Browsing by Author "Rijith, J."

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    Estimation of out-crossing rate in West Coast Tall cultivar in coconut using microsatellite markers
    (2012-11) Rajesh, M.K.; Rijith, J.; Shafeeq Rahman; Ramya, K.; Sajini, K.K.; Anitha Karun
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    Estimation of out-crossing rates in populations of West Coast Tall cultivar of coconut using microsatellite markers
    (2014-12) Rajesh, M.K.; Rijith, J.; Shafeeq Rahman; Preethi, P.; Rachana, K.E.; Sajini, K.K.; Anitha Karun
    Understanding of mating system of a plant species has fundamental importance for formulation of genetic conservation strategies and breeding programmes. The pattern of gene flow, via pollen, has a profound influence on the genetic structure within a population. Various genetic parameters, obtained from molecular marker studies, can be used to assess estimates of mating system. The aim of this study was to estimate the rate of outcrossing in West Coast Tall (WCT) cultivar of coconut, which is predominant in India, using microsatellite simple sequence repeats (SSR). Two WCT mother palms and their 88 progenies, collected as embryos for five months, were screened using 15 highly polymorphic microsatellite primers. The mating parameters were estimated using mixed mating model (MLTR software) and the extents of similarity between the mother palms and their progenies were analyzed using the NTSYS software. The percentage similarity between the mother palm and its progenies, as deduced using microsatellite data, ranged from 55 to 74 per cent. The progenies were also analyzed using a RAPD primer capable of distinguishing Tall and Dwarf palms. All the progenies were found to possess the Tall-type marker indicating that the pollen was derived from Tall palms in all the cases. The results revealed the WCT cultivar to be pre-dominantly out-crossing and indicated that proper sampling and indicated that proper sampling and breeding strategies are required to sustain the high genetic diversity found.
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    Evaluation of microsatellite markers for genetic diversity analysis among different accessions of arecanut (Areca catechu L.)
    (2012-11) Bharath, B.G.; Ananda, K.S.; Rajesh, M.K.; Nagaraja, N.R.; Rijith, J.
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    Genetic purity assessment of D x T hybrids in coconut with SSR markers
    (2012) Rajesh, M.K.; Thomas, R.J.; Rijith, J.; Shareefa, M.; Jacob, P.M.
    Assessment of genetic purity in coconut hybrids was done using molecular markers. A set of 50 hyperpolymorphic coconut SSR markers were utilized to characterize parental lines Chowghat Green Dwarf (CGD) and West Coast Tall (WCT) used for hybrid coconut production. A panel of 17 informative SSR markers capable of distinguishing these parental lines was identified and these markers were utilized in DxT hybrid seedling purity assessments in coconut nurseries.
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    Studies on genetic relationships and diversity in arecanut (Areca catechu L.) germplasm utilizing RAPD markers
    (2015) Bharath, B.G.; Ananda, K.S.; Rijith, J.; Nagaraja, N.R.; Chandran, K.P.; Anitha Karun; Rajesh, M.K