Browsing by Author "Roshna, O.M."

Now showing 1 - 4 of 4
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    Real-time PCR technique for detection of arecanut yellow leaf disease phytoplasma
    (2014) Smita Nair; Roshna, O.M.; Soumya, V.P.; Vinayaka Hegde; Suresh Kumar, M.; Manimekalai, R.; George V. Thomas
    The Yellow Leaf Disease, with phytoplasmal etiology, is a serious disease affecting arecanut palms and causing substantial yield loss. Phytoplasmas are cell wall less, unculterable, phloem limited plant pathogens generally detected using microscopy, serology and molecular techniques. Here we report a SYBR green based real time PCR approach for detection of arecanut yellow leaf disease phytoplasma.We designed efficient primers for SYBR green based real time PCR to overcome the problems in conventional PCR. Primers QPF2/QPR2, designed from highly conserved phytoplasma 16S rRNA gene was used to amplify DNA preparation from spindle leaf tissues of symptomatic palms using real time PCR. A unique melting peak at 82.3±0.5 °C was observed for symptomatic arecanut samples. The PCR products were further purified, sequenced and analysed using BLASTn. The sequences showed 99 % nucleotide identity with Indian arecanut yellow leaf disease phytoplasma sequences in the database. Two representative sequences were also deposited in the Genbank database. The present study thus devised a platform for rapid and sensitive detection of phytoplasma associated with the arecanut yellow leaf disease.
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    Real-time PCR technique-based detection of coconut root (wilt) phytoplasma
    (2011-11) Manimekalai, R.; Smita Nair; Soumya, V.P.; Roshna, O.M.; George V. Thomas
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    Updated Version of PHYTODB, the First Data Warehousing and Mining Web Server for Phytoplasma Research
    (2011) Manimekalai, R.; Anoop Raj, P.; Roshna, O.M.; Anil Paul; George V. Thomas
    PHYTODB contains a repository of phytoplasma genes and proteins. It provides a unified gateway to store, search, retrieve, update information about phytoplasma and computational resources for the analysis of nucleotide and aminoacid sequence data of phytoplasma. Server facilitates to differentiate and classify new phytoplasma for taxonomic purposes. PHYTODB database was updated by dividing the whole resources into two domains: DataBanks and Tools. DataBanks serve as the storage device of all information. Functional characterization of genes and protein are done. Updated Groupidentifier tool by rearrangement of RFLP classification scheme of phytoplasma and possibilities 6 new groups based on the new tool. PhytoDB can be obtained through http://220.227.88.253/phytodb/ .