Browsing by Author "Saji, K.V."

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    Clonal multiplication of oil palm (Elaeis guineensis Jacq)
    (2007-02-08) Raju, C.R.; Sajini, K.K.; Balachandran, S.M.; Saji, K.V.; Geetha Maheshan, K.; Rajasekharan, P.E.; Geetha, L.; Bavappa, K.V.A.
    Five mm long tender leaf explants and leaf base explants taken from 3-year-old seedlings were cultured on defined media supplemented with various auxins and cytokinins. Cultures were incubated in the light. The expaints produced callus from the veins at the cut surface in 2-3 weeks on R medium containing 20 mgl-1 2,4-D and 0.1 mgl"1 BAP. Somatic embryoids developed on lowering the 2,4-D concentration and replacing it with NAA. Bipolar and tripolar embryoids originated as snow-white protuberences from die sub-surface layers of the callus. The process of germination of the somatic embryoids was similar to that of normal zygotic embryos. However, the formation of adventitious roots was enhanced with die addition of 0.01 mgl-1 IBA to the medium.
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    Field collection and in vitro germination of coconut embryos
    (2007-02) Anitha Karun; Shivashankar, S.; Sajini, K.K.; Saji, K.V.
    A method for collection and inoculation of coconut embryos directly in the field was investigated. Embryos collected from 8-11 months old nuts were surface sterilized and cultured in liquid nutrient medium, inside a portable hood made of plexiglass. The cultures were maintained at room temperature for 3 to 5 days after which they were transferred to fresh medium and stored inside the culture room. The extent of contamination at the time of shoot production was very low. These shoots formed a number of lateral roots in Y-3 medium supplemented with N A A and IB A. The study highlights the possibility of employing the field technique for successful collection of coconut germplasm as zygotic embryos and their retrieval in vitro.
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