Browsing by Author "Sajini, K.K."
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Item Biotechnological Approaches(2017) Anitha Karun; Rajesh, M.K.; Muralikrishna, K.S.; Sajini, K.K.; Chowdappa, P.Item Clonal multiplication of oil palm (Elaeis guineensis Jacq)(2007-02-08) Raju, C.R.; Sajini, K.K.; Balachandran, S.M.; Saji, K.V.; Geetha Maheshan, K.; Rajasekharan, P.E.; Geetha, L.; Bavappa, K.V.A.Five mm long tender leaf explants and leaf base explants taken from 3-year-old seedlings were cultured on defined media supplemented with various auxins and cytokinins. Cultures were incubated in the light. The expaints produced callus from the veins at the cut surface in 2-3 weeks on R medium containing 20 mgl-1 2,4-D and 0.1 mgl"1 BAP. Somatic embryoids developed on lowering the 2,4-D concentration and replacing it with NAA. Bipolar and tripolar embryoids originated as snow-white protuberences from die sub-surface layers of the callus. The process of germination of the somatic embryoids was similar to that of normal zygotic embryos. However, the formation of adventitious roots was enhanced with die addition of 0.01 mgl-1 IBA to the medium.Item Coconut(2017) Anitha Karun; Sajini, K.K.; Aparna, V.; Rajesh, M.KItem Coconut (Cocos nucifera L.) Pollen Cryopreservation(2014) Anitha Karun; Sajini, K.K.; Niral, V.; Amarnath, C.H.; Remya, P.; Rajesh, M.K.; Samsudeen, K.; Jerard, B.A.; Florent EngelmannBACKGROUND: Coconut genetic resources are threatened by pests and pathogens, naturalhazards and human activities. Cryopreservation is the only method allowing the safe and costeffectivelong-term conservation of recalcitrant seed species such as coconut. OBJECTIVE: The objective of this work was to test the effect of cryopreservation and of cryostorage duration on coconut pollen germination and fertility. MATERIALS AND METHODS: Pollen of two coconut varieties (West Coast Tall WCT and Chowghat Orange Dwarf COD ) was collected in March-May over three successive years, desiccated to 7.5% moisture content (FW) and cryopreserved by direct immersion in liquid nitrogen. RESULTS: Germination and pollen tube length (PTL) of desiccated and cryopreserved pollen were not significantly different for both WCT and COD over the three harvest months of the three consecutive years of study. Pollen germination ranged from 24 to 32% in desiccated pollen whereas it was between 26 and 29% in cryopreserved COD pollen. In the case of WCT, germination ranged from 30 to 31% in desiccated pollen, while it was between 28 and 32% in cryopreserved pollen. PTL of cryopreserved pollen ranged between 224-390 m and 226-396 m for COD and WCT, respectively. Germination of COD pollen varied between 29.0 and 44.1% after 4 years and 1.0/1.5 years cryostorage, respectively. Germination of WCT pollen did not change significantly between 0 and 6 years cryostorage, being comprised between 32 (24 h) and 40 % (1.5 years). Germination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved. Normal seed set was observed in COD and WCT palms using pollen cryostored for 6 months and 4 years. Cryopreserved pollen of five Tall and five Dwarf accessions displayed 24-31% and 25-49% germination, respectively. CONCLUSION: These results show that it is now possible to establish pollen cryobanks to contribute to coconut germplasm long-term conservation.Item Coconut Biotechnology:New Vistas(2017-12) Rajesh, M.K.; Sajini, K.K.; Muralikrishna, K.S.; Anitha KarunItem Coconut embryo culture - Protocol for germplasm collection(CPCRI, 2002-10) Anitha Karun; Parthasarathy, V.A.; Kumaran, P.M.; Iyer, R.D.; Sajini, K.K.Item Coconut plumule : Tissue with an efficient regeneration capacity(2004-06) Rajesh, M.K.; Radha, E.; Sajini, K.K.; Anitha KarunItem Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements(2017) Anitha Karun; Rajesh, M.K; Sajini, K.K.; Muralikrishna, K.S; Neema, M.; Shareefa, M.; Regi Jacob ThomasItem COCONUT(COCOS NUCIFERA L.)POLLEN CRYOPRESERVATION(2014) Anitha Karun; Sajini, K.K.; Niral, V.; Amarnath, C.H.; Remya, P.; Rajesh, M.K; Samsudeen, K; Jerard, A.; Florent EngelmannItem Comparative study of three different methods of coconut plumule extraction for embryogenic callus induction(2012-11) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut(2016) Bhavyashree, U.; Lakshmi Jayaraj, K.; Fayas, T.P.; Muralikrishna, K.S.; Sajini, K.K.; Rajesh, M.K.; Anitha KarunItem Comparitive evaluation of embryo culture protocols in coconut(2007-02-08) Anitha Karun; Sajini, K.K.; Anuradha UpadhyayFour coconut embryo culture protocols, developed from PCA, Philippines, UPLB, Philippines, IRHO, France and CPCRI, India were tried with two tall and two dwarf cultivars. There was no significant difference among the protocols for per cent embryos germinated. However, difference among the cultivars and cultivar-by-protocol interaction was significant. The in vitro growth was slow in CPCRI protocol but the survival of the plants and ex vitro establishment were good. Vitrification was noticed only in liquid medium. Least number of plants was retrieved through IRHO protocol.Item A complimentary conservation strategy for coconut (Cocos nucifera L.) through pollen cryopreservation(2012-11) Sajini, K.K.; Anitha Karun; Amarnath, C.H.; Rajesh, M.K.Item Cryopreservation of arecanut (Areca catechu L.) pollen(2017-11) Anitha Karun; Sajini, K.K.; Muralikrishna, K.S.; Rajesh, M.K.; Florent EngelmannItem Cryopreservation of Cocoa Shoot Tips Using Pre Growth Desiccation Protocol(2011) Nilosha; Bhavyashree, U.; Sajini, K.K.; Anitha KarunItem Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos after pre-growth desiccation(2006) Sajini, K.K.; Anitha Karun; Kumaran, P.M.Coconut, being a recalcitrant species with large nuts, cryopreservation is the only option for long-term conservation of coconut . genetic resources, which can provide a viable backup to field gene banks. A simple cryopreservation technique involving pregrowth desiccation using sucrose was standardized for coconut zygotic embryos. Zygotic embryos extracted from WCT variety ofc!?conut were pre-grown in I M, 2M and 3M concentrations of sucrose and thereafter subjected to liquid nitrogen exposure for 24 hours. The results showed that 1M sucrose pre-treatment was not sufficient for dehydration as it resulted in death of the embryos after cryogenic treatment. At 2M and 3M sucrose concentrations, the moisture content of the embryos was reduced to 30% and 27% with the corresponding final recovery of plantlets after cryopreservation of 20.8% and 29%, respectively.Item Cryopreservation of Coconut (Cocos Nucifera L.) Zygotic Embryos by Vitrification(2011) Sajini, K.K.; Anitha Karun; Amarnath, C.H.; Florent EngelmannThe present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.Item Cryopreservation of mature coconut embryos by desiccation method(2005) Anitha Karun; Sajini, K.K.; Parthasarathy, V.A.Item Cryopreservaton of coconut (Cocos nucifera .L) pollen(2006) Anitha Karun; Meera Nair; Sajini, K.K.; Kumaran, P.M.; Samsudheen, K.Item Design and Analysis of Coconut Embryo Culture Experiments(2003) Anitha Karun; Muralidharan, K.; Sajini, K.K.; Parthasarathy, V.A.
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