Browsing by Author "Seema, G."

Now showing 1 - 3 of 3
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    Effect of antioxidants on oil palm leaf in vitro
    (2007-02-08) Anuradha Upadhyay; Utpala Parthasarathy; Seema, G.; Anitha Karun; Parthasarathy, V.A.
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    An efficient method of DNA extraction from coconut leaves
    (2007-02-08) Anuradha Upadhyay; Parthasarathy, V.A.; Seema, G.; Anitha Karun
    A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion.
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    An efficient method of DNA extraction from coconut leaves
    (1999) Anuradha Upadhyay; Parthasarathy, V.A.; Seema, G.; Anitha Karun
    A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion