Browsing by Author "Sergio Polakof"

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    Interaction of short-term Testosterone Treatment with Osmotic Acclimation in the Gilthead Sea Bream sparus Auratus
    (Springer-Verlag, 2008) Francisco J. Arjona; Susana Sangiao-Alvarellos; Sergio Polakof; Angel Garcia-Lopez; Maria P. Martin del Rio; Gonzalo Martinez-Rodriguez; Jose L. Soengas; Juan M. Mancera
    To Assess the Interaction between Testosterone (t) Treatment and Acclimation to Different Salinities, seawater-acclimated Gilthead Sea Bream (sparus Auratus) were Implanted with slow-release Coconut Oil Implants alone (control) or Containing T (5 µg/g Body Mass).After 5 Days, Eight Fish of Control and T-treated Groups were Sampled.The same Day, Eight Fish of each Group were Transferred to Low Salinity Water (lsw, 6 Ppt, Hypoosmotic Test), Seawater (sw, 38 Ppt, Control Test) and High Salinity Water (hsw, 55 Ppt, Hyperosmotic Test) and Sampled 9 Days Later.Gill Na+, K+-ATPase Activity Increased in HSW-acclimated Fish with Respect to SW- and LSW-acclimated Fish in both Control and T-treated Groups.Kidney Na+, K+-ATPase Activity was also Enhanced in HSW-acclimated Fish, but only in T-treated Group.From a Metabolic Point of View, most of the Changes Observed can be Attributed to the Action of Salinity and T Treatment Alone, since few Interactions between T Treatment and Osmotic Acclimation to Different Salinities were Observed.Those Interactions Included in Treated Fish:In the Liver, Decreased Capacity in Using Glucose in Fish Acclimated to Extreme Salinities;In the Gills, Decreased Capacity in Using Amino Acids in Hsw;In the Kidneys Increased Capacity in Using Amino Acids in Extreme Salinities; And in the Brain, Decreased Glycogen and Acetoacetate Levels of Fish in Lsw.