Browsing by Author "Shieh-Ping Wu"

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    Plant regeneration through shoot formation from callus of Areca catechu
    (2003) Shieh-Ping Wu; Jen-Tsung Chen; Mei-Chun Lin; Wei-Chin Chang; Hsiang-Chih Wang
    Plantlet formation through shoot formation from callus of the important palm species Areca catechu L. is described. Greenish soft callus was formed from shoot tip explants of Areca catechu L. within 4 weeks, when cultured on Gelrite-gelled MS (Murashige and Skoog, 1962) basal medium (BM) supplemented with BA (0.2 mg 1 1 l ) plus TDZ (0, 0.02 and 0.2 mg l ). The highest percentage of callus formation (100%) was found on the 1 1 medium supplemented with 0.2 mg l BA and 0.2 mg l TDZ. During subculture on the same medium for callus induction, most of calluses proliferated and 50–60% formed shoots. About 90% of shoots formed roots on BM 1 containing 0.1 mg l NAA after 4 weeks in culture. Regeneration of plantlets from shoot tips via primary callus production and a two-step process of organogenesis, required about 20 weeks.
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    Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of areca catechu l. (arecaceae)
    (Society for In Vitro Biology, 2003) Hsiang-Chih Wang; Shieh-Ping Wu; Jen-Tsung Chen; Mei-Chun Lin; Wei-Chin Chang
    An in vitro culture procedure was established for somatic embryogenesis and plant regeneration from callus cultures of the important palm ‘betel nut’ (Areca catechu L.). Segments of zygotic embryos of Areca catechu L. were cultured on Murashige and Skoog basal medium supplemented with dicamba (9.05, 18.1, 27.15, and 36.2mM). After 7–8 wk in darkness, wounded regions of explants formed callus with yellow, soft, glutinous structures. Proliferation and maintenance of callus was on the same dicamba-containing medium. With regular subculture every 8 wk, the callus showed pale yellow, compact and nodular structures. During subculture, somatic embryos were formed spontaneously from nodular callus tissues within 2–4 mo. The embryos developed into plantlets after 10 wk of culture on basal medium free of plant growth regulators. After subculturing every month for 3 mo., the plantlets were transferred to containers for acclimatization in the greenhouse. The survival rate was 24%.