Browsing by Author "Siril, E.A."

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    In Vitro Embryo Retrieval Technique for Arecanut (Areca Catechu Linn.)
    (2002) Anitha Karun; Siril, E.A.; Radha, E.; Parthasarathy, V.A.
    An in vitro germination technique for the rapid germination of arecanut embryos has been developed. Seven-month-old embryos collected from four varieties viz. Mangala, Sumangala, Sree Mangala and South Kanara Local were cultured in vitro on agar gelled hormone free EeuewensY3, + strength MS or Full strength MS medium. Embryos cultured in Y3 medium supplemented with 3% sucrose showed maximum (93.3%) germination. Varietal differences with respect to germination was also recorded and showed Sumangala and South Kanara local were most responsive. In vitro seedlings after 8 weeks in germination media were transferred to liquid media containing reduced (1.5%) sucrose for the development of roots and expansion of leaves. Fully developed in vitro seedlings are being acclimatized. Developed protocol will serve as a basis for the future in vitro conservation (cryopreservation) studies on arecanut and is also useful in the areas of safe and convenient germplasm movement, rare embryo rescue etc.
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    Somatic embryogenesis and plantlet regeneration from leaf and inflorescence explants of arecanut(Areca catechu L.)
    (2004-06) Anitha Karun; Siril, E.A.; Radha, E.; Parthasarathy, V.A.