Browsing by Author "Stumpf, P.K."

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    Fatty Acid Biosynthesis in the Developing Endosperm of Cocos nucifera
    (2007) Oo, K.C.; Stumpf, P.K.
    Endosperm tissue of developing coconut endosperm incorporated [14C] acetate and [14C]-malonate into [14C]Cg-C]8 fatty acids. The distribution of [14C] label into the various fatty acids mimicked the distribution of endogenous fatty acids at early and middle stages of endosperm development. Although I14C]C8-C18 fatty acids were taken up rapidly by endosperm tissue slices, no elongation occurred; [)4C]stearic acid was not desaturated to oleic. Cell free preparations have also been obtained from this tissue that readily incorporated [ I4C] malonyl-CoA into a range of [14C] fatty acids in the presence of ACP and NADH at pH 7.0. Employing this system, a number of experiments were designed to determine the mechanism of chain length termination. In contrast 11 intact tissue slice experiments, cell-free extracts yielded as principal products palmitic and stearic acid, although up to 20% were shorter chain acids, A number of possible mechanisms for chain length termination were proposed and tested.