Browsing by Author "Talavera, C."

Now showing 1 - 3 of 3
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    Effect of medium sucrose on the photosynthetic capacity of coconut vitroplants formed from zygotic embryos
    (2007-02-08) Santamaria, J.M.; Talavera, C.; Lavergne, D.; Trabelsi, S.; Verdeil, J.L.; Huet, C.; Rival, A.; Hamon, S.; Nato, A.
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    High irradiance can minimize the negative effect of exogenous sucrose on the photosynthetic capacity of in vitro grown coconut plantlets
    (2005) Fuentes, G.; Talavera, C.; Desjardins, Y.; Santamaria, J.M.
    There is increasing evidence that the sucrose normally added to the culture medium affects negatively the photosynthetic capacity of plantlets. At the same time, however, sucrose cannot be eliminated from the medium, as it is required for normal in vitro growth. We argue that this is true only under the conventional light conditions of growth-rooms. In the present paper irradiance of growth-rooms was increased 10 times and although the sucrose-inhibitory effect was found at high sucrose concentrations, it was possible to grow coconut (Cocos nucifera L.) plantlets without sucrose. Those plantlets showed both high photosynthetic capacity and comparable in vitro growth to those grown with sucrose in the medium under conventional growth-room irradiance. Nevertheless, the best growth was achieved under mixotrophic conditions where at high irradiance and moderate sucrose concentrations plantlets accumulated 27 % more biomass than plantlets grown without sucrose under high irradiance and 43 and 73 % more biomass than their counterparts at low irradiance with or without sucrose, respectively.
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    Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis
    (1998) Chan, J.L.; Saenz, L.; Talavera, C.; Hornung, R.; Robert, M.; Oropeza, C.
    A protocol was developed for coconut regeneration using plumules from mature zygotic embryos as explants, and media with the synthetic growth regulators 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. Evidence for the regeneration process from these tissues occurring through somatic embryogenesis is presented. The somatic embryos were capable of germination, subsequent development into plantlets and successful transfer to the nursery. The yields were larger, nearly twofold for calli and over tenfold for calli bearing somatic embryos, than those previously reported with inflorescence explants. The present protocol thus represents an improvement in time and yield over previous protocols. Even though plumule explants are not the ideal tissue source due to possible genetic heterogeneity, the improvements made here may be applicable to tissues from mature plants. In addition, micropropagation of coconut using plumules is potentially useful when they are obtained from fruit produced from selected parents of outstanding performance, such as those resistant to diseases.