Browsing by Author "Valérie Hocher"

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    Cryopreservation by encapsulation–dehydration of plumules of coconut (Cocos nucifera L.)
    (2008) Oulo N’Nan; Valérie Hocher; Jean-Luc Verdeil; Jean-Louis Konan; Koffi Ballo; Fanja Mondeil; Bernard Malaurie
    This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.