Browsing by Author "Wetmore, R.H."

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Fern callus tissue culture
    (1951-02) Morel, G.; Wetmore, R.H.
    Spores of Osmunda cinnamomea L. germinated on Knudson's medium developed into the usual heart-shaped, thin, preen prothalli. Five produced sreen calluses on the upper surfaces after 2 nmnths. These calluses grew slowly or died on this medium. When transferred to the same medium supplemented by B-vitamins in appropriate concentration the calluses grew rapidly and continue to do so. The calluses were of nodular masses of larpe-opllrd parenchyma in the center of each of which jp an islet, of meristematic tissue. Certain of the older-parenchyma cells form tracheids. Chromosomal studies show the prothailial callus to be haploid. It is suggesled that the presence of traohean tissue in the independent prothalli of vascular cryptogams is therefore not by itself sufficient evidence that apogamy is an accompanying phenomenon. Onh one callus has so far been experimentally induced on prothalli of Osmunda
  • Thumbnail Image
    Item
    Tissue Culture of Monocotyledons
    (1951-02) Morel, G.; Wetmore, R.H.
    It has been possible to obtain the proliferation in vitro of tissue of monocotyledons for potentially indefinite periods. Our studies seem to indicate that these tissues require certain growth substances, as yet undetermined, which are found in the milk of immature coconuts.