Browsing by Author "Wetten, A.C."

Now showing 1 - 4 of 4
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    The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees
    (2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
    Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSS V-infected Ameionado cocoa trees (T 1 , T2 and T4). The virps was transmitted to primary somatic embryos induced from the i nfecied callus tissues atiherace of 10 (J9%), 18 (14%) and 16 (15%) forTl.T2 and T4, re«,p
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    Transmission of cocoa swollen shoot virus by seeds
    (Elsevier, 2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
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    Transmission of cocoa swollen shoot virus by seeds
    (2008) Quainoo, A.K.; Wetten, A.C.; Allainguillaume, J.
    A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. FCR/captllary electrophoresis. using cocoa swollen shoot virus primers designed from the most conserved regions of the six published cocoa genome sequences, allowed the detection of cocoa swollen shoot virus in jil the component parts of cocoa seeds from cocoa swollen shoot virus-infected trees. PCR/capiHary electrop horesis revealed the presence of cocoa swollen shoot virus in seedlings raised from seeds obtained from cocoa swollen shoot virus-infected trees. The high frequency with which the virus was transmitted through the seedlings suggested that cocoa swollen shoot virus is transmitted by seeds. This has serious implications for cocoa germplasm conservation and distribution.