Coconut-Tissue culture

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Browsing Coconut-Tissue culture by Subject "Androgenesis"

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    Androgenic potential in coconut (Cocos nucifera L.)
    (2008) Verdeil, J.L.; Hocher, V.; Perera, P.I.P.; Yakandawala, D.M.D.; Bandupriya, H.D.D.; Weerakoon, L.K.
    Conditions for induction of androgenesis in coconut cv. Sri Lanka Tall were studied. Anthers collected from inflorescences at four maturity stages were given heat (38 C) or cold (4 C) pretreatments for 1, 3, 6 and 14 days, either prior to or post inoculation. Three different basal media and different anther densities were also tested. Androgenesis was observed only in anthers collected from inflorescences 3 weeks before splitting (WBS) and after a heat pretreatment at 38 C for 6 days. Modified Eeuwens Y3 liquid medium supplemented with 100 lM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.1% activated charcoal and 9% sucrose was effective in inducing an androgenic response. The lowest anther density tested, 10 per petri plate, was found to be the optimal density. When androgenic calli or embryos were subcultured to Y3 medium containing 66 lM 2,4-D, followed by transfer to Y3 medium without plant growth regulators and finally to Y3 medium containing 5 lM 6-benzyladenine (BA) and 0.35 lM gibberellic acid (GA3), plantlets regenerated at a frequency of 7%. Histological study indicated that the calli and embryos originated from the inner tissues of the anthers. Ploidy analysis of calli and embryos showed that they were haploid. This is the first report of successful androgenesis yielding haploid plants from coconut anthers
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    Use of SSR markers to determine the anther-derived homozygous lines in coconut
    (2008) Perera, P.I.P.; Verdeil, J.L.; Hocher, V.; Perera, L.; Yakandawala, D.M.D.; Weerakoon, L.K.
    Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs